Autophagy in Caenorhabditis elegans.

Tímea Sigmond, János Barna, Márton L. Tóth, K. Takács-Vellai, Gabriella Pásti, A. Kovács, T. Vellai

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Autophagy (cellular self-eating) is a highly regulated, lysosome-mediated catabolic process of eukaryotic cells to segregate by a special membrane and subsequently degrade their own constituents during development or starvation. Electron microscopy analysis reveals autophagic elements in various cell types of the nematode Caenorhabditis elegans, whose genome contains counterparts of several yeast genes involved in autophagy. Genetic manipulation inactivating autophagy-related genes in C. elegans causes defects in development, affects dauer larval morphogenesis, accelerates aging thereby shortening life span, reduces cell size, decreases survival during starvation, promotes apoptotic cell death, and protects neurons from undergoing hyperactive ion channel- or neurotoxin-induced degeneration. These results implicate autophagy in various developmental and cellular functions such as reproductive growth, aging, and cell growth, as well as cell survival and loss. This chapter discusses methods of inactivating C. elegans autophagy genes by RNA interference, testing the resistance of autophagy-deficient nematodes to starvation-induced stress, handling mutants carrying a deletion in the autophagy pathway, and monitoring autophagic activity by using LysoTracker Red dye or reporters labeled with green fluorescent protein. Such methods may be adaptable to identify additional roles of autophagy in development and cellular function, and may also help to detect the intracellular accumulation of autophagy proteins and monitor autophagosome formation.

Original languageEnglish
Pages (from-to)521-540
Number of pages20
JournalMethods in Enzymology
Volume451
Publication statusPublished - Jan 1 2009

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Autophagy
Caenorhabditis elegans
Genes
Aging of materials
Starvation
Neurotoxins
Cell growth
Cell death
Green Fluorescent Proteins
Ion Channels
Yeast
Electron microscopy
Neurons
Coloring Agents
Cells
RNA
Membranes
Defects
Monitoring
Testing

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Sigmond, T., Barna, J., Tóth, M. L., Takács-Vellai, K., Pásti, G., Kovács, A., & Vellai, T. (2009). Autophagy in Caenorhabditis elegans. Methods in Enzymology, 451, 521-540.

Autophagy in Caenorhabditis elegans. / Sigmond, Tímea; Barna, János; Tóth, Márton L.; Takács-Vellai, K.; Pásti, Gabriella; Kovács, A.; Vellai, T.

In: Methods in Enzymology, Vol. 451, 01.01.2009, p. 521-540.

Research output: Contribution to journalArticle

Sigmond, T, Barna, J, Tóth, ML, Takács-Vellai, K, Pásti, G, Kovács, A & Vellai, T 2009, 'Autophagy in Caenorhabditis elegans.', Methods in Enzymology, vol. 451, pp. 521-540.
Sigmond T, Barna J, Tóth ML, Takács-Vellai K, Pásti G, Kovács A et al. Autophagy in Caenorhabditis elegans. Methods in Enzymology. 2009 Jan 1;451:521-540.
Sigmond, Tímea ; Barna, János ; Tóth, Márton L. ; Takács-Vellai, K. ; Pásti, Gabriella ; Kovács, A. ; Vellai, T. / Autophagy in Caenorhabditis elegans. In: Methods in Enzymology. 2009 ; Vol. 451. pp. 521-540.
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