Spontaneous autophagocytosis was observed in mouse seminal vesicle cells during incubation for 2 h in vitro. The number of autophagic vacuoles formed was greatest at 37° C and decreased when the temperature was lowered. At 22° C it reached the near-zero value characteristic of nonincubated control cells. Incubation of the cells at 37° C in the presence of 0.1 mg/ml vinblastine sulfate resulted in a marked increase in the number of autophagic vacuoles, but the drug was ineffective at 22° C. Puromycin (10-3 M) exerted no influence on spontaneous autophagocytosis, but cycloheximide in concentrations from 10-7M to 10-3M inhibited both spontaneous and vinblastine-induced autophagocytosis. The formation of tubulin paracrystals in vinblastine treated cells was not prevented either by low (22° C) temperature or in the presence of cycloheximide.
|Number of pages||8|
|Journal||Virchows Archiv B Cell Pathology Including Molecular Pathology|
|Publication status||Published - Dec 1 1980|
- protein synthesis inhibitors
- seminal vesicle
ASJC Scopus subject areas
- Pathology and Forensic Medicine