Autophagocytosis in mouse seminal vesicle cells in vitro - Temperature dependence and effects of vinblastine and inhibitors of protein synthesis

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Abstract

Spontaneous autophagocytosis was observed in mouse seminal vesicle cells during incubation for 2 h in vitro. The number of autophagic vacuoles formed was greatest at 37° C and decreased when the temperature was lowered. At 22° C it reached the near-zero value characteristic of nonincubated control cells. Incubation of the cells at 37° C in the presence of 0.1 mg/ml vinblastine sulfate resulted in a marked increase in the number of autophagic vacuoles, but the drug was ineffective at 22° C. Puromycin (10-3 M) exerted no influence on spontaneous autophagocytosis, but cycloheximide in concentrations from 10-7M to 10-3M inhibited both spontaneous and vinblastine-induced autophagocytosis. The formation of tubulin paracrystals in vinblastine treated cells was not prevented either by low (22° C) temperature or in the presence of cycloheximide.

Original languageEnglish
Pages (from-to)97-104
Number of pages8
JournalVirchows Archiv B Cell Pathology Including Molecular Pathology
Volume32
Issue number1
DOIs
Publication statusPublished - Dec 1 1980

Keywords

  • autophagocytosis
  • protein synthesis inhibitors
  • seminal vesicle
  • temperature
  • vinblastine

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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