Automated on-line dispersive liquid-liquid microextraction based on a sequential injection system

Vasil Andruch, Carolina Cecilia Acebal, Jana Škrlíková, Hana Sklenářová, Petr Solich, I. Balogh, F. Billes, Lívia Kocúrová

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

A novel approach for sequential injection-dispersive liquid-liquid microextraction (SI-DLLME) has been suggested. The method is based on the aspiration and mixing of a sample and all required aqueous reagents in the holding coil of an SIA system, delivering it into a conical tube and adding in a mixture of extraction solvent, auxiliary solvent and disperser solvent at high flow rate, resulting in the formation of a cloudy state and the extraction of an analyte. The mixture of extraction and auxiliary solvent is immiscible with water and has a density significantly higher than that of water; consequently, the resulting fine droplets in the mixture, which contain the extracted analyte, are self-sedimented in a short time at the bottom of conical tube. Thus, no centrifugation and no use of a microcolumn are required for separation of the extraction phase. Afterwards, the extracted analyte is aspirated and transferred to a micro-volume Z-flow cell, and the absorbance is measured.The performance of the suggested approach is demonstrated by the SI-DLLME of thiocyanate ions in the form of ion associate with dimethylindocarbocyanine reagent, followed by spectrophotometric detection. A mixture of amyl acetate (as extraction solvent), tetrachloromethane (as auxiliary solvent) and acetonitrile (as disperser solvent) was selected for the DLLME procedure. The appropriate experimental conditions for conventional DLLME and automated SI-DLLME were investigated. The analytical performance of both these procedures was compared. The absorbance of the colored extracts at wavelength 555nm obeys Beer's law in the range of 3.13-28.2 for conventional DLLME and 0.29-5.81mgL-1 of SCN for SI-DLLME, and the limit of detection, calculated from a blank test based on 3s, is 0.110 for conventional DLLME and 0.017mgL-1 for SI-DLLME.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalMicrochemical Journal
Volume100
Issue number1
DOIs
Publication statusPublished - Jan 2012

Fingerprint

Liquids
Solvent extraction
Centrifugation
Water
Carbon Tetrachloride
Flow rate
Ions
Wavelength

Keywords

  • Sequential injection-dispersive liquid-liquid microextraction (SI-DLLME)
  • Thiocyanate
  • UV-vis spectrophotometry

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Andruch, V., Acebal, C. C., Škrlíková, J., Sklenářová, H., Solich, P., Balogh, I., ... Kocúrová, L. (2012). Automated on-line dispersive liquid-liquid microextraction based on a sequential injection system. Microchemical Journal, 100(1), 77-82. https://doi.org/10.1016/j.microc.2011.09.006

Automated on-line dispersive liquid-liquid microextraction based on a sequential injection system. / Andruch, Vasil; Acebal, Carolina Cecilia; Škrlíková, Jana; Sklenářová, Hana; Solich, Petr; Balogh, I.; Billes, F.; Kocúrová, Lívia.

In: Microchemical Journal, Vol. 100, No. 1, 01.2012, p. 77-82.

Research output: Contribution to journalArticle

Andruch, V, Acebal, CC, Škrlíková, J, Sklenářová, H, Solich, P, Balogh, I, Billes, F & Kocúrová, L 2012, 'Automated on-line dispersive liquid-liquid microextraction based on a sequential injection system', Microchemical Journal, vol. 100, no. 1, pp. 77-82. https://doi.org/10.1016/j.microc.2011.09.006
Andruch, Vasil ; Acebal, Carolina Cecilia ; Škrlíková, Jana ; Sklenářová, Hana ; Solich, Petr ; Balogh, I. ; Billes, F. ; Kocúrová, Lívia. / Automated on-line dispersive liquid-liquid microextraction based on a sequential injection system. In: Microchemical Journal. 2012 ; Vol. 100, No. 1. pp. 77-82.
@article{c8d7c903c3d745d7a8b6bcb9cb36fbf3,
title = "Automated on-line dispersive liquid-liquid microextraction based on a sequential injection system",
abstract = "A novel approach for sequential injection-dispersive liquid-liquid microextraction (SI-DLLME) has been suggested. The method is based on the aspiration and mixing of a sample and all required aqueous reagents in the holding coil of an SIA system, delivering it into a conical tube and adding in a mixture of extraction solvent, auxiliary solvent and disperser solvent at high flow rate, resulting in the formation of a cloudy state and the extraction of an analyte. The mixture of extraction and auxiliary solvent is immiscible with water and has a density significantly higher than that of water; consequently, the resulting fine droplets in the mixture, which contain the extracted analyte, are self-sedimented in a short time at the bottom of conical tube. Thus, no centrifugation and no use of a microcolumn are required for separation of the extraction phase. Afterwards, the extracted analyte is aspirated and transferred to a micro-volume Z-flow cell, and the absorbance is measured.The performance of the suggested approach is demonstrated by the SI-DLLME of thiocyanate ions in the form of ion associate with dimethylindocarbocyanine reagent, followed by spectrophotometric detection. A mixture of amyl acetate (as extraction solvent), tetrachloromethane (as auxiliary solvent) and acetonitrile (as disperser solvent) was selected for the DLLME procedure. The appropriate experimental conditions for conventional DLLME and automated SI-DLLME were investigated. The analytical performance of both these procedures was compared. The absorbance of the colored extracts at wavelength 555nm obeys Beer's law in the range of 3.13-28.2 for conventional DLLME and 0.29-5.81mgL-1 of SCN for SI-DLLME, and the limit of detection, calculated from a blank test based on 3s, is 0.110 for conventional DLLME and 0.017mgL-1 for SI-DLLME.",
keywords = "Sequential injection-dispersive liquid-liquid microextraction (SI-DLLME), Thiocyanate, UV-vis spectrophotometry",
author = "Vasil Andruch and Acebal, {Carolina Cecilia} and Jana Škrl{\'i}kov{\'a} and Hana Sklen{\'a}řov{\'a} and Petr Solich and I. Balogh and F. Billes and L{\'i}via Koc{\'u}rov{\'a}",
year = "2012",
month = "1",
doi = "10.1016/j.microc.2011.09.006",
language = "English",
volume = "100",
pages = "77--82",
journal = "Microchemical Journal",
issn = "0026-265X",
publisher = "Elsevier Inc.",
number = "1",

}

TY - JOUR

T1 - Automated on-line dispersive liquid-liquid microextraction based on a sequential injection system

AU - Andruch, Vasil

AU - Acebal, Carolina Cecilia

AU - Škrlíková, Jana

AU - Sklenářová, Hana

AU - Solich, Petr

AU - Balogh, I.

AU - Billes, F.

AU - Kocúrová, Lívia

PY - 2012/1

Y1 - 2012/1

N2 - A novel approach for sequential injection-dispersive liquid-liquid microextraction (SI-DLLME) has been suggested. The method is based on the aspiration and mixing of a sample and all required aqueous reagents in the holding coil of an SIA system, delivering it into a conical tube and adding in a mixture of extraction solvent, auxiliary solvent and disperser solvent at high flow rate, resulting in the formation of a cloudy state and the extraction of an analyte. The mixture of extraction and auxiliary solvent is immiscible with water and has a density significantly higher than that of water; consequently, the resulting fine droplets in the mixture, which contain the extracted analyte, are self-sedimented in a short time at the bottom of conical tube. Thus, no centrifugation and no use of a microcolumn are required for separation of the extraction phase. Afterwards, the extracted analyte is aspirated and transferred to a micro-volume Z-flow cell, and the absorbance is measured.The performance of the suggested approach is demonstrated by the SI-DLLME of thiocyanate ions in the form of ion associate with dimethylindocarbocyanine reagent, followed by spectrophotometric detection. A mixture of amyl acetate (as extraction solvent), tetrachloromethane (as auxiliary solvent) and acetonitrile (as disperser solvent) was selected for the DLLME procedure. The appropriate experimental conditions for conventional DLLME and automated SI-DLLME were investigated. The analytical performance of both these procedures was compared. The absorbance of the colored extracts at wavelength 555nm obeys Beer's law in the range of 3.13-28.2 for conventional DLLME and 0.29-5.81mgL-1 of SCN for SI-DLLME, and the limit of detection, calculated from a blank test based on 3s, is 0.110 for conventional DLLME and 0.017mgL-1 for SI-DLLME.

AB - A novel approach for sequential injection-dispersive liquid-liquid microextraction (SI-DLLME) has been suggested. The method is based on the aspiration and mixing of a sample and all required aqueous reagents in the holding coil of an SIA system, delivering it into a conical tube and adding in a mixture of extraction solvent, auxiliary solvent and disperser solvent at high flow rate, resulting in the formation of a cloudy state and the extraction of an analyte. The mixture of extraction and auxiliary solvent is immiscible with water and has a density significantly higher than that of water; consequently, the resulting fine droplets in the mixture, which contain the extracted analyte, are self-sedimented in a short time at the bottom of conical tube. Thus, no centrifugation and no use of a microcolumn are required for separation of the extraction phase. Afterwards, the extracted analyte is aspirated and transferred to a micro-volume Z-flow cell, and the absorbance is measured.The performance of the suggested approach is demonstrated by the SI-DLLME of thiocyanate ions in the form of ion associate with dimethylindocarbocyanine reagent, followed by spectrophotometric detection. A mixture of amyl acetate (as extraction solvent), tetrachloromethane (as auxiliary solvent) and acetonitrile (as disperser solvent) was selected for the DLLME procedure. The appropriate experimental conditions for conventional DLLME and automated SI-DLLME were investigated. The analytical performance of both these procedures was compared. The absorbance of the colored extracts at wavelength 555nm obeys Beer's law in the range of 3.13-28.2 for conventional DLLME and 0.29-5.81mgL-1 of SCN for SI-DLLME, and the limit of detection, calculated from a blank test based on 3s, is 0.110 for conventional DLLME and 0.017mgL-1 for SI-DLLME.

KW - Sequential injection-dispersive liquid-liquid microextraction (SI-DLLME)

KW - Thiocyanate

KW - UV-vis spectrophotometry

UR - http://www.scopus.com/inward/record.url?scp=81955164234&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=81955164234&partnerID=8YFLogxK

U2 - 10.1016/j.microc.2011.09.006

DO - 10.1016/j.microc.2011.09.006

M3 - Article

VL - 100

SP - 77

EP - 82

JO - Microchemical Journal

JF - Microchemical Journal

SN - 0026-265X

IS - 1

ER -