Automated N-sGlycosylation analysis for translational Glycomics

QUO vadis?

Research output: Contribution to journalArticle

Abstract

The glycome is the entire set of sugars in a cell, tissue or organism at a certain time, including free and complex forms. Unlike genomes, the glycome is highly dynamic, due to the interplay of those more than 600 enzymes that can be involved in the complex pathways of protein glycosylation. In addition, glycosylation is cell, protein and site specific and epigenetic factors regulating the expression of glycosyltransferases and glycosidases may represent further diversifying mechanisms, influencing the functions of the proteins to which carbohydrates are attached. In the biomedical field, altered glycosylation is frequently associated with pathological conditions. Glycosylation modulation in the biotechnology industry, usually due to changes in bioprocessing/cell culture conditions, may alter the affectivity of biotherapeutics. In both instances, information about specific glycosylation motifs such as the type and number of sugar monomers along with their position and linkage specificity are of high importance. Glycomics, as a subset of glycobiology, systematically studies all glycan structures in a given sample. To fulfill the need of such global glycomics studies, automated, high throughput (preferably in 96 well plate formats) and robust bioanalytical platforms are required to address sample preparation (glycoprotein capture, glycan release and carbohydrate labeling), separation (capillary electrophoresis or liquid chromatography) and data processing (glycoinformatics) issues. This talk will confer the state of the art of analytical glycomics and discuss recent efforts and future prospective of this emerging field in regards to system integration, translational options and their implications in the biomedical and biopharmaceutical arena.

Original languageEnglish
JournalChemicke Listy
Volume107
Issue number3
Publication statusPublished - 2013

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Glycosylation
Sugars
Polysaccharides
Carbohydrates
Capillary electrophoresis
Glycosyltransferases
Proteins
Glycoside Hydrolases
Liquid chromatography
Biotechnology
Cell culture
Labeling
Glycoproteins
Genes
Monomers
Throughput
Modulation
Glycomics
Tissue
Enzymes

ASJC Scopus subject areas

  • Chemistry(all)

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Automated N-sGlycosylation analysis for translational Glycomics : QUO vadis? / Guttman, A.

In: Chemicke Listy, Vol. 107, No. 3, 2013.

Research output: Contribution to journalArticle

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