Ultrathin-layer agarose gel electrophoresis combines the advantages of the multilane format of conventional slab and the high performance capabilities of capillary gel electrophoresis. This article describes the detection of separated sample components in real-time by using an integrated scanning laser-induced fluorescence-avalanche photodiode detection system. Ultrathin-layer agarose gel electrophoresis is a novel and high performance separation technique that provides a simple platform for fast deoxyribonucleic acid fragment analysis with the advantage that the same gel-filled cassette can be used simultaneously for a size range of 20-23,130 base pairs. The author provides examples of rapid genotyping and high-throughput mutation analysis of polymerase chain reaction-generated fragments.
|Number of pages||7|
|Journal||LCGC North America|
|Publication status||Published - Nov 1999|
ASJC Scopus subject areas
- Analytical Chemistry