Automated determination of levodopa and carbidopa in plasma by high-performance liquid chromatography-electrochemical detection using an on-line flow injection analysis sample pretreatment unit

A. Tolokán, I. Klebovich, K. Balogh-Nemes, G. Horvai

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

An automated analytical procedure is described for the parallel determination of L-3,4-dihydroxyphenylalanine (levodopa, L-dopa, LD) and the analogous hydrazine compound carbidopa (CD) in dog plasma by ion-pair high-performance liquid chromatography with electrochemical detection (HPLC-ED). After deproteinization of the plasma samples with perchloric acid the catecholamines were extracted from the supernatant by adsorption on a small column filled with alumina. The extraction and redissolution were automatically performed in a flow injection analysis unit (FIA) coupled to the HPLC system. The performance of the whole system was tested on dog plasma samples including specimens taken after oral administration of the anti-Parkinsonism drug Duellin®, which is a combination tablet of levodopa and carbidopa.

Original languageEnglish
Pages (from-to)201-207
Number of pages7
JournalJournal of Chromatography B: Biomedical Applications
Volume698
Issue number1-2
DOIs
Publication statusPublished - Sep 26 1997

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High performance liquid chromatography
Levodopa
hydrazine
Plasmas
Carbidopa
Aluminum Oxide
Tablets
Catecholamines
Ions
Adsorption
Pharmaceutical Preparations
levodopa drug combination carbidopa

Keywords

  • Carbidopa
  • Levodopa

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

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AU - Klebovich, I.

AU - Balogh-Nemes, K.

AU - Horvai, G.

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