Automated carbohydrate profiling by capillary electrophoresis: A bioindustrial approach

Julia Khandurina, David L. Blum, Justin T. Stege, András Guttman

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Automated, high-resolution, quantitative, high-throughput analysis of mono- and oligosaccharides, produced by enzymatic digestion of cellohexaose (model substrate) and lignocellulosic biomass, is demonstrated using high-performance capillary electrophoresis in conjunction with a single-step fluorophore labeling strategy for sensitive laser-induced fluorescence detection. Unattended batch sample processing from 96-well plates enabled reliable industrial-scale carbohydrate analysis. Excellent resolution of mono- and oligosaccharides was achieved under suppressed electroosmotic flow conditions, using either covalently or dynamically coated fused-silica capillary columns. The proposed approach readily supports the demands of bioindustrial operation environments with respect to high-throughput carbohydrate profiling.

Original languageEnglish
Pages (from-to)2326-2331
Number of pages6
JournalELECTROPHORESIS
Volume25
Issue number14
DOIs
Publication statusPublished - Jul 1 2004

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Keywords

  • Biotechnology
  • Carbohydrate profiling
  • High-performance capillary electrophoresis
  • Laser-induced fluorescence
  • Lignocellulosic biomass degradation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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