Autolytic activation and localization in Schneider cells (S2) of calpain B from Drosophila

Attila Farkas, Peter Tompa, Éva Schád, Rita Sinka, Gáspár Jékely, P. Friedrich

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Calpain B is one of the two calpain homologues in Drosophila melanogaster that are proteolytically active. We studied its activation by Ca2+ both in vitro and in vivo, in Schneider (S2) cells. Activation involves the autolytic cleavage, at two major sites, of the N-terminal segment, the length of which was earlier underestimated. Site-directed mutagenesis at the autolytic sites did not prevent autolysis, but only shifted its sites. Calpain B mRNA was detectable in all developmental stages of the fly. In situ hybridization and immunostaining showed expression in ovaries, embryo and larvae, with high abundance in larval salivary glands. In S2 cells, calpain B was mainly in the cytoplasm and upon a rise in Ca2+ the enzyme adhered to intracellular membranes.

Original languageEnglish
Pages (from-to)299-305
Number of pages7
JournalBiochemical Journal
Volume378
Issue number2
DOIs
Publication statusPublished - Mar 1 2004

Fingerprint

Calpain
Chemical activation
Autolysis
Intracellular Membranes
Mutagenesis
Site-Directed Mutagenesis
Salivary Glands
Drosophila melanogaster
Diptera
In Situ Hybridization
Larva
Ovary
Cytoplasm
Embryonic Structures
Membranes
Messenger RNA
Drosophila CalpB protein
Enzymes

Keywords

  • Autolysis
  • Calpain
  • Drosophila
  • Expression pattern
  • Translocation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Autolytic activation and localization in Schneider cells (S2) of calpain B from Drosophila. / Farkas, Attila; Tompa, Peter; Schád, Éva; Sinka, Rita; Jékely, Gáspár; Friedrich, P.

In: Biochemical Journal, Vol. 378, No. 2, 01.03.2004, p. 299-305.

Research output: Contribution to journalArticle

Farkas, Attila ; Tompa, Peter ; Schád, Éva ; Sinka, Rita ; Jékely, Gáspár ; Friedrich, P. / Autolytic activation and localization in Schneider cells (S2) of calpain B from Drosophila. In: Biochemical Journal. 2004 ; Vol. 378, No. 2. pp. 299-305.
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