Autolysis and aging of Penicillium chrysogenum cultures under carbon starvation: Chitinase production and antifungal effect of allosamidin

László Sámi, Tünde Pusztahelyi, Tamás Emri, Zoltán Varecza, Andrea Fekete, Ágnes Grallert, Zsolt Karányi, László Kiss, István Pócsi

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

In carbon-depleted cultures of Penicillium chrysogenum, age-related chitinases were shown to play a crucial role in both autolysis and fragmentation as indicated by in vivo enzyme inhibition experiments using allosamidin. This pseudotrisaccharide even hindered significantly the outgrowth of new hyphal tips from the surviving yeastlike fragments after glucose supplementation. The antifungal effect of allosamidin on autolyzing P. chrysogenum mycelia was fungistatic rather than fungicidal. In growing hyphae, membrane-bound microsomal chitinase zymogen(s) were detected, which may be indicative of some compartmentalization of these hydrolases. Later, during autolysis, no zymogenic chitinase was detected in any enzyme fraction studied, including microsomes. These observations may explain the different sensitivity of growing and autolyzing mycelia to allosamidin. Chitinases taking part in the age-related fragmentation of hyphae and the outgrowth of surviving hyphal fragments seem to be potent targets for future antifungal drug research.

Original languageEnglish
Pages (from-to)201-211
Number of pages11
JournalJournal of General and Applied Microbiology
Volume47
Issue number4
DOIs
Publication statusPublished - Jan 1 2001

Keywords

  • Allosamidin
  • Autolysis
  • Chitinase
  • Cryptic growth
  • Glutathione
  • Hyphal fragmentation
  • Penicillium chrysogenum
  • Zymogen activation

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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