AUTEN-67 (Autophagy Enhancer-67) Hampers the Progression of Neurodegenerative Symptoms in a Drosophila model of Huntington's Disease

Viktor Billesa, Tibor Kovacs, Bernadette Hotzi, Anna Manzeger, Kinga Tagscherer, Marcell Komlos, Anna Tarnocia, Zsolt Padar, Attila Erdos, Annamaria Bjelik, Adam Legradi, K. Gulya, Balazs Gulyas, T. Vellai

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: Autophagy, a lysosome-mediated self-degradation process of eukaryotic cells, serves as a main route for the elimination of cellular damage [1-3]. Such damages include aggregated, oxidized or misfolded proteins whose accumulation can cause various neurodegenerative pathologies, including Huntington's disease (HD). Objective: Here we examined whether enhanced autophagic activity can alleviate neurophatological features in a Drosophila model of HD (the transgenic animals express a human mutant Huntingtin protein with a long polyglutamine repeat, 128Q). Methods: We have recently identified an autophagy-enhancing small molecule, AUTEN-67 (autophagy enhancer 67), with potent neuroprotective effects [4]. AUTEN-67 was applied to induce autophagic activity in the HD model used in this study. Results:We showed that AUTEN-67 treatment interferes with the progressive accumulation of ubiquitinated proteins in the brain of Drosophila transgenic for the pathological 128Q form of human Huntingtin protein. The compound significantly improved the climbing ability and moderately extended the mean life span of these flies. Furthermore, brain tissue samples from human patients diagnosed for HD displayed increased levels of the autophagy substrate SQSTM1/p62 protein, as compared with controls. Conclusions: These results imply that AUTEN-67 impedes the progression of neurodegenerative symptoms characterizing HD, and that autophagy is a promising therapeutic target for treating this pathology. In humans, AUTEN-67 may have the potential to delay the onset and decrease the severity of HD.

Original languageEnglish
Pages (from-to)133-147
Number of pages15
JournalJournal of Huntington's disease
Volume5
Issue number2
DOIs
Publication statusPublished - 2016

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Keywords

  • autophagy, AUTEN-67
  • climbing assay
  • Drosophila
  • Huntingtin
  • Huntington's disease
  • neurodegeneration
  • polyq
  • Ref(2)P/SQSTM1/p62
  • ubiquitinated proteins

ASJC Scopus subject areas

  • Medicine(all)
  • Clinical Neurology
  • Cellular and Molecular Neuroscience

Cite this

Billesa, V., Kovacs, T., Hotzi, B., Manzeger, A., Tagscherer, K., Komlos, M., Tarnocia, A., Padar, Z., Erdos, A., Bjelik, A., Legradi, A., Gulya, K., Gulyas, B., & Vellai, T. (2016). AUTEN-67 (Autophagy Enhancer-67) Hampers the Progression of Neurodegenerative Symptoms in a Drosophila model of Huntington's Disease. Journal of Huntington's disease, 5(2), 133-147. https://doi.org/10.3233/JHD-150180