Augmented biogas production from protein-rich substrates and associated metagenomic changes

Etelka Kovács, Roland Wirth, Gergely Maróti, Zoltán Bagi, K. Nagy, J. Mináróvits, G. Rákhely, K. Kovács

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

This study demonstrates that appropriate adaptation of the microbial community to protein-rich biomass can lead to sustainable biogas production. The process of acclimation to these unusual mono-substrates was controlled by the protease activity of the microbial community. Meat extract (C/N=3.32) and kitchen waste (C/N=12.43) were used as biogas substrates. Metagenome analysis highlighted several mesophilic strains that displayed a preference for protein degradation. Bacillus coagulans, Bacillus subtilis and Pseudomonas fluorescens were chosen for detailed investigation. Pure cultures were added to biogas reactors fed solely with protein-rich substrates. The bioaugmentation resulted in a 50% increase in CH4 production even without any acclimation. The survival and biological activity of the added bacteria were followed in fed-batch fermenters by qPCR. Stable biogas production was observed for an extended period of time in laboratory CSTR reactors fed with biomass of low C/N.

Original languageEnglish
Pages (from-to)254-261
Number of pages8
JournalBioresource Technology
Volume178
DOIs
Publication statusPublished - Feb 1 2015

Fingerprint

Metagenomics
Biofuels
Biogas
biogas
Proteins
substrate
protein
Acclimatization
Bacilli
Substrates
acclimation
Biomass
microbial community
Metagenome
Fermenters
Pseudomonas fluorescens
Kitchens
Meats
biomass
Bioactivity

Keywords

  • Anaerobic degradation
  • Bioaugmentation
  • Biogas
  • Metagenomics
  • Protein-rich waste

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Waste Management and Disposal
  • Medicine(all)

Cite this

Augmented biogas production from protein-rich substrates and associated metagenomic changes. / Kovács, Etelka; Wirth, Roland; Maróti, Gergely; Bagi, Zoltán; Nagy, K.; Mináróvits, J.; Rákhely, G.; Kovács, K.

In: Bioresource Technology, Vol. 178, 01.02.2015, p. 254-261.

Research output: Contribution to journalArticle

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