Augmentation of macrophage candidactdal function by recombinant human granulocyte-monocyte colony stimulating factor (rhgm-csf) and myeloperoxidase (rhMPO)

L. Máródi, C. Tournay, R. Kâposzta, N. Moguilevsky

Research output: Contribution to journalArticle

Abstract

We found earlier that monocytes, rich in MPO, killed C.albjcans at a significantly higher rate and degree than did monocyte-derived macrophages known to lack MPO (J.ImmuQol. 146:2783). We hypothesized, therefore, that the capacity of macrophages to kill Candida might be retained in the presence of MPO. In this study, the ability of rhMPO to augment the fungicidal activity of resident and rhGM-CSF-activated macrophages for C.albicans was evaluated. rhMPO was produced in transfected and amplified Chinese hamster ovary cells and purified by a combination of ion exchange and metalchelate chromatography. Addition of rhMPO (concentrations: 0.8, 1.6, 3.2, and 6.4 U/ml) to suspensions of resident macrophages and serum opsonized C.albicans resulted in significant increases in the killing capacity of Candida at concentrations of 3.2 U/ml and 6.4 U/ml (p6 resident and activated cells, respectively; 17±2 % and 32±4% killing by resident and activated cells, respectively), with no appreciable increase being achieved by treatment of cells with 300 to 1000 U/ml rhGM-CSF. Addition of rhMPO (concentrations: 0.8 to 6.4) to the phagocytix mixture further increased the candidacidalcapacity of rhGM-CSF-activated macrophages (p<O.OSat each concentrations used). These data indicate that exogenous rhMPO augments candidacidal capacity of both resident and rhGM-CSF-activated macrophages with a more profound effect on activated cells. These data suggest that rhMPO might be effective in the treatment of invasive candidiasis.

Original languageEnglish
JournalJournal of Investigative Medicine
Volume44
Issue number3
Publication statusPublished - 1996

Fingerprint

Colony-Stimulating Factors
Macrophages
Granulocyte Colony-Stimulating Factor
Peroxidase
Monocytes
Candida
Cells
Invasive Candidiasis
Ion Exchange Chromatography
Chromatography
Cricetulus
Ovary
Ion exchange
Suspensions
Serum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

@article{2a8b5fb6e3844af6a3b3ff7573d436f2,
title = "Augmentation of macrophage candidactdal function by recombinant human granulocyte-monocyte colony stimulating factor (rhgm-csf) and myeloperoxidase (rhMPO)",
abstract = "We found earlier that monocytes, rich in MPO, killed C.albjcans at a significantly higher rate and degree than did monocyte-derived macrophages known to lack MPO (J.ImmuQol. 146:2783). We hypothesized, therefore, that the capacity of macrophages to kill Candida might be retained in the presence of MPO. In this study, the ability of rhMPO to augment the fungicidal activity of resident and rhGM-CSF-activated macrophages for C.albicans was evaluated. rhMPO was produced in transfected and amplified Chinese hamster ovary cells and purified by a combination of ion exchange and metalchelate chromatography. Addition of rhMPO (concentrations: 0.8, 1.6, 3.2, and 6.4 U/ml) to suspensions of resident macrophages and serum opsonized C.albicans resulted in significant increases in the killing capacity of Candida at concentrations of 3.2 U/ml and 6.4 U/ml (p6 resident and activated cells, respectively; 17±2 {\%} and 32±4{\%} killing by resident and activated cells, respectively), with no appreciable increase being achieved by treatment of cells with 300 to 1000 U/ml rhGM-CSF. Addition of rhMPO (concentrations: 0.8 to 6.4) to the phagocytix mixture further increased the candidacidalcapacity of rhGM-CSF-activated macrophages (p<O.OSat each concentrations used). These data indicate that exogenous rhMPO augments candidacidal capacity of both resident and rhGM-CSF-activated macrophages with a more profound effect on activated cells. These data suggest that rhMPO might be effective in the treatment of invasive candidiasis.",
author = "L. M{\'a}r{\'o}di and C. Tournay and R. K{\^a}poszta and N. Moguilevsky",
year = "1996",
language = "English",
volume = "44",
journal = "Journal of Investigative Medicine",
issn = "1081-5589",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Augmentation of macrophage candidactdal function by recombinant human granulocyte-monocyte colony stimulating factor (rhgm-csf) and myeloperoxidase (rhMPO)

AU - Máródi, L.

AU - Tournay, C.

AU - Kâposzta, R.

AU - Moguilevsky, N.

PY - 1996

Y1 - 1996

N2 - We found earlier that monocytes, rich in MPO, killed C.albjcans at a significantly higher rate and degree than did monocyte-derived macrophages known to lack MPO (J.ImmuQol. 146:2783). We hypothesized, therefore, that the capacity of macrophages to kill Candida might be retained in the presence of MPO. In this study, the ability of rhMPO to augment the fungicidal activity of resident and rhGM-CSF-activated macrophages for C.albicans was evaluated. rhMPO was produced in transfected and amplified Chinese hamster ovary cells and purified by a combination of ion exchange and metalchelate chromatography. Addition of rhMPO (concentrations: 0.8, 1.6, 3.2, and 6.4 U/ml) to suspensions of resident macrophages and serum opsonized C.albicans resulted in significant increases in the killing capacity of Candida at concentrations of 3.2 U/ml and 6.4 U/ml (p6 resident and activated cells, respectively; 17±2 % and 32±4% killing by resident and activated cells, respectively), with no appreciable increase being achieved by treatment of cells with 300 to 1000 U/ml rhGM-CSF. Addition of rhMPO (concentrations: 0.8 to 6.4) to the phagocytix mixture further increased the candidacidalcapacity of rhGM-CSF-activated macrophages (p<O.OSat each concentrations used). These data indicate that exogenous rhMPO augments candidacidal capacity of both resident and rhGM-CSF-activated macrophages with a more profound effect on activated cells. These data suggest that rhMPO might be effective in the treatment of invasive candidiasis.

AB - We found earlier that monocytes, rich in MPO, killed C.albjcans at a significantly higher rate and degree than did monocyte-derived macrophages known to lack MPO (J.ImmuQol. 146:2783). We hypothesized, therefore, that the capacity of macrophages to kill Candida might be retained in the presence of MPO. In this study, the ability of rhMPO to augment the fungicidal activity of resident and rhGM-CSF-activated macrophages for C.albicans was evaluated. rhMPO was produced in transfected and amplified Chinese hamster ovary cells and purified by a combination of ion exchange and metalchelate chromatography. Addition of rhMPO (concentrations: 0.8, 1.6, 3.2, and 6.4 U/ml) to suspensions of resident macrophages and serum opsonized C.albicans resulted in significant increases in the killing capacity of Candida at concentrations of 3.2 U/ml and 6.4 U/ml (p6 resident and activated cells, respectively; 17±2 % and 32±4% killing by resident and activated cells, respectively), with no appreciable increase being achieved by treatment of cells with 300 to 1000 U/ml rhGM-CSF. Addition of rhMPO (concentrations: 0.8 to 6.4) to the phagocytix mixture further increased the candidacidalcapacity of rhGM-CSF-activated macrophages (p<O.OSat each concentrations used). These data indicate that exogenous rhMPO augments candidacidal capacity of both resident and rhGM-CSF-activated macrophages with a more profound effect on activated cells. These data suggest that rhMPO might be effective in the treatment of invasive candidiasis.

UR - http://www.scopus.com/inward/record.url?scp=33749435627&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33749435627&partnerID=8YFLogxK

M3 - Article

VL - 44

JO - Journal of Investigative Medicine

JF - Journal of Investigative Medicine

SN - 1081-5589

IS - 3

ER -