We have started to elaborate a general transformation and plant regeneration system for Beta vulgaris L. using combined gene transfer procedure. Three methods were tested: particle bombardment with pure wolfram micro-carriers prior to Agrobacterium treatment, vortexing and infiltration of explants in Agrobacterium suspension, halving of explants. The histochemical analysis of transient and stabile gus-gene expression showed the beneficial effect of explant halving and infiltration in Agrobacterium suspension.
|Number of pages||2|
|Journal||Acta Biologica Szegediensis|
|Publication status||Published - Sep 5 2002|
- Agrobacterium tumefaciens
- Beta vulgaris L.
- Combined gene transfer
- Gus-gene expression
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Agricultural and Biological Sciences(all)