Attempts to immunize chickens with Cryptosporidium baileyi oocyst extract

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In order to study the possibility of immunization against Cryptosporidium baileyi with extracted crude antigen, Arbor Acres chickens were injected intramuscularly with 80 μg of C. baileyi oocyst-derived proteins (uninfected immunized, UI) or inoculated orally with 8 x 105 viable C. baileyi oocysts (infected control, IC) at 1 wk of age. The immunization was repeated in the UI group at 2 wk of age. Uninfected (UC) birds served as controls. All animals in UI, IC, and UC groups were challenged orally with 8 x 105 C. baileyi oocysts at the age of 4 wk. Blood samples were collected when birds were 4 and 6 wk of age, and sera were examined by enzyme-linked immunosorbent assay for the presence of antibodies against C. baileyi. Total oocyst output of UI chickens was about 60% of that of UC birds after challenge, and the prepatent and patent periods were nearly identical in the latter 2 groups. In contrast, IC birds developed complete resistance to challenge infections. These results suggest that immunization with the oocyst extract of C. baileyi may confer some degree of protection against oral challenge; however, the protection is less effective than that induced by primary oral infection. The lack of significant difference between the antibody responses of IC and UI animals to C. baileyi at 4 wk of age suggests that serum antibodies play little role in acquired resistance to challenge infection.

Original languageEnglish
Pages (from-to)650-652
Number of pages3
JournalJournal of Parasitology
Volume82
Issue number4
Publication statusPublished - Aug 1996

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Cryptosporidium baileyi
Cryptosporidium
Oocysts
oocysts
Chickens
Birds
immunization
chickens
extracts
antibody
bird
Immunization
serum
Infection
blood serum
antibodies
birds
mouth
Antibodies
animal

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Parasitology
  • Microbiology

Cite this

Attempts to immunize chickens with Cryptosporidium baileyi oocyst extract. / Hornok, S.; Sréter, T.; Békési, L.; Széll, Z.; Varga, I.

In: Journal of Parasitology, Vol. 82, No. 4, 08.1996, p. 650-652.

Research output: Contribution to journalArticle

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