Attack of Microcystis aeruginosa bloom on a Ceratophyllum submersum field

Ecotoxicological measurements in real environment with real microcystin exposure

Andrea Zsuzsanna Ujvárosi, Milán Riba, Tamás Garda, G. Gyémánt, György Vereb, Márta M-Hamvas, G. Vasas, Csaba Máthé

Research output: Contribution to journalArticle

Abstract

Overproduction of toxic cyanobacteria is a type of harmful algal blooms (HABs). The heptapeptide microcystins (MCs) are one of the most common cyanotoxins. There is increasing research concerning the effects of MCs on growth and physiology of vascular plants, however there is a lack of studies on their direct effects on aquatic macrophytes in the real environment. Here we report the occurrence of a MC producing HAB in Lake Bárdos, Hungary in 2012 with harmful effects on cytological, histological and biochemical parameters of Ceratophyllum submersum (soft hornwort) plants naturally growing at the blooming site. Blue-Green Sinapis Test (BGST) showed high toxicity of HAB samples. Cell-free water samples contained a significant amount of MCs (7.31 ± 0.17 μg L−1) while C. submersum plants contained 1.01 ± 0.21 μg g DW−1 MCs. Plants showed significant increases of protein content and decreases of anthocyanin content and carotenoid/chlorophyll ratio, indicating physiological stress- as compared to plants from the control (MC free) sampling site of the same water body. Histological and cytological studies showed (i) radial swelling and the abnormal formation of lateral buds at the shoot tip leading to abnormal development; (ii) the fragmentation of nuclei as well as accumulation of phenolics in the nucleus indicating that the HAB induced cell death and stress reactions at the nuclear level. The most relevant effect was the increase of histone H3 phosphorylation in metaphase chromosomes: since MCs are strong inhibitors of protein phosphatases, this alteration is related to the biochemical targets of these toxins. The HAB decreased peroxidase activity, but increased nuclease and protease activities, showing the decreased capacity of plants to face biotic stress and as the cytological changes, the induction of cell death. This study is one of the first to show the complex harmful changes in aquatic plants that co-exist with HABs.

Original languageEnglish
Pages (from-to)735-745
Number of pages11
JournalScience of the Total Environment
Volume662
DOIs
Publication statusPublished - Apr 20 2019

Fingerprint

Microcystins
Cell death
algal bloom
Anthocyanins
Proteins
Phosphorylation
Phosphatases
Physiology
Chlorophyll
Chromosomes
Toxicity
Swelling
Lakes
Water
Sampling
protein
aquatic plant
carotenoid
vascular plant
bud

Keywords

  • Cell death
  • Ceratophyllum submersum
  • HAB
  • Histone H3 phosphorylation
  • Microcystins
  • Real exposure

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution

Cite this

Attack of Microcystis aeruginosa bloom on a Ceratophyllum submersum field : Ecotoxicological measurements in real environment with real microcystin exposure. / Ujvárosi, Andrea Zsuzsanna; Riba, Milán; Garda, Tamás; Gyémánt, G.; Vereb, György; M-Hamvas, Márta; Vasas, G.; Máthé, Csaba.

In: Science of the Total Environment, Vol. 662, 20.04.2019, p. 735-745.

Research output: Contribution to journalArticle

Ujvárosi, Andrea Zsuzsanna ; Riba, Milán ; Garda, Tamás ; Gyémánt, G. ; Vereb, György ; M-Hamvas, Márta ; Vasas, G. ; Máthé, Csaba. / Attack of Microcystis aeruginosa bloom on a Ceratophyllum submersum field : Ecotoxicological measurements in real environment with real microcystin exposure. In: Science of the Total Environment. 2019 ; Vol. 662. pp. 735-745.
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