Attachment and proliferation of human osteoblast-like cells (MG-63) on laser-ablated titanium implant material

Ágnes Györgyey, Krisztina Ungvári, Gabriella Kecskeméti, Judit Kopniczky, Béla Hopp, Albert Oszkó, István Pelsöczi, Zoltán Rakonczay, Katalin Nagy, Kinga Turzó

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45 Citations (Scopus)

Abstract

Demand is increasing for shortening the long (3-6 months) osseointegration period to rehabilitate patients' damaged chewing apparatus in as short a time as possible. For dental implants, as for biomaterials in general, the bio- and osseointegration processes can be controlled at molecular and cellular levels by modification of the implant surface. One of the most promising of such surface modifications is laser ablation, as demonstrated by our previous results [46]. Commercially pure (CP4) sand-blasted, acid-etched titanium disks (Denti® System Ltd., Hungary) were irradiated with a KrF excimer laser (248 nm, fluence 0.4 J/cm2, FWHM 18 ns, 2000 pulses), or with a Nd:YAG laser (532 nm, 1.3 J/cm2, 10 ns, 200 pulses) then examined by SEM, AFM, and XPS. In vitro attachment (24 h) and proliferation (72 h) of MG-63 osteoblast cells were investigated via dimethylthiazol-diphenyl tetrazolium bromide (MTT), alamarBlue (AB) assays alkaline phosphatase quantification (ALP) and SEM. SEM and AFM revealed significant changes in morphology and roughness. XPS confirmed the presence of TiO2 on each sample; after Nd:YAG treatment a reduced state of Ti (Ti3 +) was also observed. MTT, AB and ALP measurements detected an increase in the number of cells between the 24- and 72 hour observations; however, laser treatment did not affect cell attachment and proliferation significantly.

Original languageEnglish
Pages (from-to)4251-4259
Number of pages9
JournalMaterials Science and Engineering C
Volume33
Issue number7
DOIs
Publication statusPublished - Oct 1 2013

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Keywords

  • Cell attachment and proliferation
  • Laser ablation
  • MG-63 osteoblasts
  • Surface modification
  • Titanium implant

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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