Atrazine resistance entails a limited xanthophyll cycle activity, a lower PSII efficiency and an altered pattern of excess excitation dissipation

Gyula Váradi, Hilda Polyánka, Éva Darkó, Endre Lehoczki

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Abstract

Atrazine-resistant (AR) weeds have a modified D1 protein structure, with a Ser264→Gly mutation on the D1 protein, near the plastoquinone binding niche. The photosynthetic performance, the light response of the xanthophyll cycle and chlorophyll fluorescence quenching-related parameters were compared in attached leaves of susceptible (S) and AR biotypes of the C3 dicot Chenopodium album L., Epilobium adenocaulon Hausskn., Erigeron canadensis L., Senecio vuigaris L. and Solanum nigrum L. and the C4 dicot Amaranthus retroflexus L. grown under natural high-light conditions. No significant difference in CO2 assimilation rate per leaf area unit was found between the S and AR biotypes of the investigated C3 plants, whereas the AR biotype of A. retroflexus exhibited a relatively poor photosynthetic performance. The D1 protein mutant plants expressed a reduced activity of light-stimulated zeaxanthin formation. Neither the lower violaxanthin de-epoxidase activity nor the depletion of ascorbate seems to be the cause of the lower in vivo zeaxanthin formation in the AR plants. All the D1 mutant weeds had limited light-induced non-photochemical (NPQ) and photochemical (qp) quenching capacities, and displayed a higher photosensitivity, as characterized by the ratio (1-qp)/NPQ and a higher susceptibility to photoinhibition. Analysis of the chlorophyll fluorescence parameters showed that a lower proportion of excitation energy was allocated to PSII photochemistry, while a higher excess of excitation remained in the AR weeds relative to the S plants.

Original languageEnglish
Pages (from-to)47-56
Number of pages10
JournalPhysiologia Plantarum
Volume118
Issue number1
DOIs
Publication statusPublished - May 1 2003

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ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

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