ATP utilization for calcium uptake and force production in different types of human skeletal muscle fibres

P. Szentesi, R. Zaremba, W. Van Mechelen, G. J.M. Stienen

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

1. The contractile properties and ATPase activity of skinned human skeletal muscle fibres from vastus lateralis were examined. Fibre types were resolved from single fibre segments by SDS-polyacrylamide gel electrophoresis. ATPase activity was determined by enzymatic coupling of ATP resynthesis to the oxidation of NADH. 2. The partitioning of ATPase activity into (a) calcium-activated activity due to actomyosin (AM) interaction, (b) calcium-activated activity of the sarcoplasmic reticular (SR) calcium pump, and (c) basal (calcium independent) activity was investigated by comparing ATP utilization before and after exposure of the preparations for 30 min to a solution containing 0.5% Triton X-100, which effectively abolished the SR ATPase activity. 3. Partitioning of ATPase activity was also determined by measuring ATP utilization and force at different concentrations of butanedione monoxime (BDM), which inhibits AM interaction. 4. The results obtained with Triton X-100 and BDM were similar. At saturating Ca2+ concentrations and 20 °C, the AM, SR and basal ATPase activities per litre cell volume (± S.E.M.) amounted to 46 ± 4, 51 ± 4 and 19 ± 2 μ Ms-1 in type I fibres (n = 21), 139 ± 14, 69 ± 8 and 30 ± 3 μ Ms-1 in type IIA fibres (n = 25), 137 ± 22, 175 ± 28 and 26 ± 8 μ Ms-1 in type IIA/B fibres (n = 4) and 108 ± 13, 169 ± 42 and 32 ± 8 μ Ms-1 in type IIB fibres (n = 2). 5. These results indicate that ATP utilization for SR Ca2+ pumping in fast fibres is considerably larger than in slow fibres. The SR ATPase activity in human muscle represents a considerable fraction of the total (AM + SR + basal) ATPase activity.

Original languageEnglish
Pages (from-to)393-403
Number of pages11
JournalJournal of Physiology
Volume531
Issue number2
DOIs
Publication statusPublished - Mar 1 2001

ASJC Scopus subject areas

  • Physiology

Fingerprint Dive into the research topics of 'ATP utilization for calcium uptake and force production in different types of human skeletal muscle fibres'. Together they form a unique fingerprint.

  • Cite this