Atmospheric pressure chemical ionization mass spectrometry of aldehydes in biological matrices

Kornél Nagy, Ferenc Pollreisz, Zoltán Takáts, K. Vékey

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Application of high-performance liquid chromatography (HPLC) and tandem mass spectrometry (MS/MS) for detection of aldehydes in biological samples such as blood, yoghurt, and milk, is reported. Sample preparation is easy, and the presented method is both sensitive and selective. It is based on the widely used dinitrophenylhydrazine derivatization, followed by extraction with n-hexane and a simple reversed-phase HPLC separation. Detection is performed by atmospheric pressure chemical ionization (APCI) in negative ion mode, with detection limits in the low picogram range. Using MS/MS, acetone and propionaldehyde can clearly be distinguished, facilitating propionaldehyde quantitation even in the presence of high acetone levels. Quantitation by direct MS/MS is also feasible, well suited for high-throughput applications, although not as accurate as using HPLC/MS/MS.

Original languageEnglish
Pages (from-to)2473-2478
Number of pages6
JournalRapid Communications in Mass Spectrometry
Volume18
Issue number20
DOIs
Publication statusPublished - 2004

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High performance liquid chromatography
Aldehydes
Atmospheric pressure
Ionization
Mass spectrometry
Acetone
Blood
Negative ions
Throughput
propionaldehyde

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

Atmospheric pressure chemical ionization mass spectrometry of aldehydes in biological matrices. / Nagy, Kornél; Pollreisz, Ferenc; Takáts, Zoltán; Vékey, K.

In: Rapid Communications in Mass Spectrometry, Vol. 18, No. 20, 2004, p. 2473-2478.

Research output: Contribution to journalArticle

Nagy, Kornél ; Pollreisz, Ferenc ; Takáts, Zoltán ; Vékey, K. / Atmospheric pressure chemical ionization mass spectrometry of aldehydes in biological matrices. In: Rapid Communications in Mass Spectrometry. 2004 ; Vol. 18, No. 20. pp. 2473-2478.
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