Atg17/FIP200 localizes to perilysosomal Ref(2)P aggregates and promotes autophagy by activation of Atg1 in Drosophila

Péter Nagy, Manuéla Kárpáti, Ágnes Varga, Karolina Pircs, Zsolt Venkei, Szabolcs Takáts, Kata Varga, Balázs Érdi, Krisztina Hegedus, G. Juhász

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Phagophore-derived autophagosomes deliver cytoplasmic material to lysosomes for degradation and reuse. Autophagy mediated by the incompletely characterized actions of Atg proteins is involved in numerous physiological and pathological settings including stress resistance, immunity, aging, cancer, and neurodegenerative diseases. Here we characterized Atg17/FIP200, the Drosophila ortholog of mammalian RB1CC 1/FIP200, a proposed functional equivalent of yeast Atg17. Atg17 disruption inhibits basal, starvation-induced and developmental autophagy, and interferes with the programmed elimination of larval salivary glands and midgut during metamorphosis. Upon starvation, Atg17-positive structures appear at aggregates of the selective cargo Ref(2)P/p62 near lysosomes. This location may be similar to the perivacuolar PAS (phagophore assembly site) described in yeast. Drosophila Atg17 is a member of the Atg1 kinase complex as in mammals, and we showed that it binds to the other subunits including Atg1, Atg13, and Atg101 (C12orf44 in humans, 9430023L20Rik in mice and RGD1359310 in rats). Atg17 is required for the kinase activity of endogenous Atg1 in vivo, as loss of Atg17 prevents the Atg1-dependent shift of endogenous Atg13 to hyperphosphorylated forms, and also blocks punctate Atg1 localization during starvation. Finally, we found that Atg1 overexpression induces autophagy and reduces cell size in Atg17-null mutant fat body cells, and that overexpression of Atg17 promotes endogenous Atg13 phosphorylation and enhances autophagy in an Atg1-dependent manner in the fat body. We propose a model according to which the relative activity of Atg1, estimated by the ratio of hyper-to hypophosphorylated Atg13, contributes to setting low (basal) vs. high (starvation-induced) autophagy levels in Drosophila.

Original languageEnglish
Pages (from-to)453-467
Number of pages15
JournalAutophagy
Volume10
Issue number3
DOIs
Publication statusPublished - 2014

Fingerprint

Autophagy
Drosophila
Starvation
Fat Body
Lysosomes
Phosphotransferases
Yeasts
Salivary Glands
Cell Size
Adipocytes
Neurodegenerative Diseases
Mammals
Immunity
Phosphorylation
Neoplasms
Proteins

Keywords

  • Atg1
  • Atg13
  • Atg17/FIP201
  • Autophagy
  • Drosophila
  • Lysosome
  • Ref(2)P/p62
  • TOR

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Atg17/FIP200 localizes to perilysosomal Ref(2)P aggregates and promotes autophagy by activation of Atg1 in Drosophila. / Nagy, Péter; Kárpáti, Manuéla; Varga, Ágnes; Pircs, Karolina; Venkei, Zsolt; Takáts, Szabolcs; Varga, Kata; Érdi, Balázs; Hegedus, Krisztina; Juhász, G.

In: Autophagy, Vol. 10, No. 3, 2014, p. 453-467.

Research output: Contribution to journalArticle

Nagy, P, Kárpáti, M, Varga, Á, Pircs, K, Venkei, Z, Takáts, S, Varga, K, Érdi, B, Hegedus, K & Juhász, G 2014, 'Atg17/FIP200 localizes to perilysosomal Ref(2)P aggregates and promotes autophagy by activation of Atg1 in Drosophila', Autophagy, vol. 10, no. 3, pp. 453-467. https://doi.org/10.4161/auto.27442
Nagy, Péter ; Kárpáti, Manuéla ; Varga, Ágnes ; Pircs, Karolina ; Venkei, Zsolt ; Takáts, Szabolcs ; Varga, Kata ; Érdi, Balázs ; Hegedus, Krisztina ; Juhász, G. / Atg17/FIP200 localizes to perilysosomal Ref(2)P aggregates and promotes autophagy by activation of Atg1 in Drosophila. In: Autophagy. 2014 ; Vol. 10, No. 3. pp. 453-467.
@article{f91a434024974946911d9631b44a597b,
title = "Atg17/FIP200 localizes to perilysosomal Ref(2)P aggregates and promotes autophagy by activation of Atg1 in Drosophila",
abstract = "Phagophore-derived autophagosomes deliver cytoplasmic material to lysosomes for degradation and reuse. Autophagy mediated by the incompletely characterized actions of Atg proteins is involved in numerous physiological and pathological settings including stress resistance, immunity, aging, cancer, and neurodegenerative diseases. Here we characterized Atg17/FIP200, the Drosophila ortholog of mammalian RB1CC 1/FIP200, a proposed functional equivalent of yeast Atg17. Atg17 disruption inhibits basal, starvation-induced and developmental autophagy, and interferes with the programmed elimination of larval salivary glands and midgut during metamorphosis. Upon starvation, Atg17-positive structures appear at aggregates of the selective cargo Ref(2)P/p62 near lysosomes. This location may be similar to the perivacuolar PAS (phagophore assembly site) described in yeast. Drosophila Atg17 is a member of the Atg1 kinase complex as in mammals, and we showed that it binds to the other subunits including Atg1, Atg13, and Atg101 (C12orf44 in humans, 9430023L20Rik in mice and RGD1359310 in rats). Atg17 is required for the kinase activity of endogenous Atg1 in vivo, as loss of Atg17 prevents the Atg1-dependent shift of endogenous Atg13 to hyperphosphorylated forms, and also blocks punctate Atg1 localization during starvation. Finally, we found that Atg1 overexpression induces autophagy and reduces cell size in Atg17-null mutant fat body cells, and that overexpression of Atg17 promotes endogenous Atg13 phosphorylation and enhances autophagy in an Atg1-dependent manner in the fat body. We propose a model according to which the relative activity of Atg1, estimated by the ratio of hyper-to hypophosphorylated Atg13, contributes to setting low (basal) vs. high (starvation-induced) autophagy levels in Drosophila.",
keywords = "Atg1, Atg13, Atg17/FIP201, Autophagy, Drosophila, Lysosome, Ref(2)P/p62, TOR",
author = "P{\'e}ter Nagy and Manu{\'e}la K{\'a}rp{\'a}ti and {\'A}gnes Varga and Karolina Pircs and Zsolt Venkei and Szabolcs Tak{\'a}ts and Kata Varga and Bal{\'a}zs {\'E}rdi and Krisztina Hegedus and G. Juh{\'a}sz",
year = "2014",
doi = "10.4161/auto.27442",
language = "English",
volume = "10",
pages = "453--467",
journal = "Autophagy",
issn = "1554-8627",
publisher = "Landes Bioscience",
number = "3",

}

TY - JOUR

T1 - Atg17/FIP200 localizes to perilysosomal Ref(2)P aggregates and promotes autophagy by activation of Atg1 in Drosophila

AU - Nagy, Péter

AU - Kárpáti, Manuéla

AU - Varga, Ágnes

AU - Pircs, Karolina

AU - Venkei, Zsolt

AU - Takáts, Szabolcs

AU - Varga, Kata

AU - Érdi, Balázs

AU - Hegedus, Krisztina

AU - Juhász, G.

PY - 2014

Y1 - 2014

N2 - Phagophore-derived autophagosomes deliver cytoplasmic material to lysosomes for degradation and reuse. Autophagy mediated by the incompletely characterized actions of Atg proteins is involved in numerous physiological and pathological settings including stress resistance, immunity, aging, cancer, and neurodegenerative diseases. Here we characterized Atg17/FIP200, the Drosophila ortholog of mammalian RB1CC 1/FIP200, a proposed functional equivalent of yeast Atg17. Atg17 disruption inhibits basal, starvation-induced and developmental autophagy, and interferes with the programmed elimination of larval salivary glands and midgut during metamorphosis. Upon starvation, Atg17-positive structures appear at aggregates of the selective cargo Ref(2)P/p62 near lysosomes. This location may be similar to the perivacuolar PAS (phagophore assembly site) described in yeast. Drosophila Atg17 is a member of the Atg1 kinase complex as in mammals, and we showed that it binds to the other subunits including Atg1, Atg13, and Atg101 (C12orf44 in humans, 9430023L20Rik in mice and RGD1359310 in rats). Atg17 is required for the kinase activity of endogenous Atg1 in vivo, as loss of Atg17 prevents the Atg1-dependent shift of endogenous Atg13 to hyperphosphorylated forms, and also blocks punctate Atg1 localization during starvation. Finally, we found that Atg1 overexpression induces autophagy and reduces cell size in Atg17-null mutant fat body cells, and that overexpression of Atg17 promotes endogenous Atg13 phosphorylation and enhances autophagy in an Atg1-dependent manner in the fat body. We propose a model according to which the relative activity of Atg1, estimated by the ratio of hyper-to hypophosphorylated Atg13, contributes to setting low (basal) vs. high (starvation-induced) autophagy levels in Drosophila.

AB - Phagophore-derived autophagosomes deliver cytoplasmic material to lysosomes for degradation and reuse. Autophagy mediated by the incompletely characterized actions of Atg proteins is involved in numerous physiological and pathological settings including stress resistance, immunity, aging, cancer, and neurodegenerative diseases. Here we characterized Atg17/FIP200, the Drosophila ortholog of mammalian RB1CC 1/FIP200, a proposed functional equivalent of yeast Atg17. Atg17 disruption inhibits basal, starvation-induced and developmental autophagy, and interferes with the programmed elimination of larval salivary glands and midgut during metamorphosis. Upon starvation, Atg17-positive structures appear at aggregates of the selective cargo Ref(2)P/p62 near lysosomes. This location may be similar to the perivacuolar PAS (phagophore assembly site) described in yeast. Drosophila Atg17 is a member of the Atg1 kinase complex as in mammals, and we showed that it binds to the other subunits including Atg1, Atg13, and Atg101 (C12orf44 in humans, 9430023L20Rik in mice and RGD1359310 in rats). Atg17 is required for the kinase activity of endogenous Atg1 in vivo, as loss of Atg17 prevents the Atg1-dependent shift of endogenous Atg13 to hyperphosphorylated forms, and also blocks punctate Atg1 localization during starvation. Finally, we found that Atg1 overexpression induces autophagy and reduces cell size in Atg17-null mutant fat body cells, and that overexpression of Atg17 promotes endogenous Atg13 phosphorylation and enhances autophagy in an Atg1-dependent manner in the fat body. We propose a model according to which the relative activity of Atg1, estimated by the ratio of hyper-to hypophosphorylated Atg13, contributes to setting low (basal) vs. high (starvation-induced) autophagy levels in Drosophila.

KW - Atg1

KW - Atg13

KW - Atg17/FIP201

KW - Autophagy

KW - Drosophila

KW - Lysosome

KW - Ref(2)P/p62

KW - TOR

UR - http://www.scopus.com/inward/record.url?scp=84892805825&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84892805825&partnerID=8YFLogxK

U2 - 10.4161/auto.27442

DO - 10.4161/auto.27442

M3 - Article

VL - 10

SP - 453

EP - 467

JO - Autophagy

JF - Autophagy

SN - 1554-8627

IS - 3

ER -