AtfA bZIP-type transcription factor regulates oxidative and osmotic stress responses in Aspergillus nidulans

Anita Balázs, Imre Pócsi, Z. Hamari, E. Leiter, T. Emri, Márton Miskei, Judit Oláh, Viktória Tóth, Nikoletta Hegedus, Rolf A. Prade, M. Molnár, I. Pócsi

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The aim of the study was to demonstrate that the bZIP-type transcription factor AtfA regulates different types of stress responses in Aspergillus nidulans similarly to Atf1, the orthologous 'all-purpose' transcription factor of Schizosaccharomyces pombe. Heterologous expression of atfA in a S. pombe Δatf1 mutant restored the osmotic stress tolerance of fission yeast in surface cultures to the same level as recorded in complementation studies with the atf1 gene, and a partial complementation of the osmotic and oxidative-stress-sensitive phenotypes was also achieved in submerged cultures. AtfA is therefore a true functional ortholog of fission yeast's Atf1. As demonstrated by RT-PCR experiments, elements of both oxidative (e.g. catalase B) and osmotic (e.g. glycerol-3-phosphate dehydrogenase B) stress defense systems were transcriptionally regulated by AtfA in a stress-type-specific manner. Deletion of atfA resulted in oxidative-stress-sensitive phenotypes while the high-osmolarity stress sensitivity of the fungus was not affected significantly. In A. nidulans, the glutathione/glutathione disulfide redox status of the cells as well as apoptotic cell death and autolysis seemed to be controlled by regulatory elements other than AtfA. In conclusion, the orchestrations of stress responses in the aspergilli and in fission yeast share several common features, but further studies are needed to answer the important question of whether a fission yeast-like core environmental stress response also operates in the euascomycete genus Aspergillus.

Original languageEnglish
Pages (from-to)289-303
Number of pages15
JournalMolecular Genetics and Genomics
Volume283
Issue number3
DOIs
Publication statusPublished - Mar 2010

Fingerprint

Basic-Leucine Zipper Transcription Factors
Osmoregulation
Aspergillus nidulans
Schizosaccharomyces
Oxidative Stress
Osmotic Pressure
Aspergillus
Glycerolphosphate Dehydrogenase
Phenotype
Autolysis
Glutathione Disulfide
Catalase
Osmolar Concentration
Oxidation-Reduction
Glutathione
Fungi
Cell Death
Transcription Factors
Polymerase Chain Reaction
Genes

Keywords

  • Apoptosis
  • Autolysis
  • Auxotrophy
  • CESR
  • Osmotic stress
  • Oxidative stress
  • Stress signaling

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

AtfA bZIP-type transcription factor regulates oxidative and osmotic stress responses in Aspergillus nidulans. / Balázs, Anita; Pócsi, Imre; Hamari, Z.; Leiter, E.; Emri, T.; Miskei, Márton; Oláh, Judit; Tóth, Viktória; Hegedus, Nikoletta; Prade, Rolf A.; Molnár, M.; Pócsi, I.

In: Molecular Genetics and Genomics, Vol. 283, No. 3, 03.2010, p. 289-303.

Research output: Contribution to journalArticle

Balázs, Anita ; Pócsi, Imre ; Hamari, Z. ; Leiter, E. ; Emri, T. ; Miskei, Márton ; Oláh, Judit ; Tóth, Viktória ; Hegedus, Nikoletta ; Prade, Rolf A. ; Molnár, M. ; Pócsi, I. / AtfA bZIP-type transcription factor regulates oxidative and osmotic stress responses in Aspergillus nidulans. In: Molecular Genetics and Genomics. 2010 ; Vol. 283, No. 3. pp. 289-303.
@article{ab8b2882e99d403cb331b91bf40d52f1,
title = "AtfA bZIP-type transcription factor regulates oxidative and osmotic stress responses in Aspergillus nidulans",
abstract = "The aim of the study was to demonstrate that the bZIP-type transcription factor AtfA regulates different types of stress responses in Aspergillus nidulans similarly to Atf1, the orthologous 'all-purpose' transcription factor of Schizosaccharomyces pombe. Heterologous expression of atfA in a S. pombe Δatf1 mutant restored the osmotic stress tolerance of fission yeast in surface cultures to the same level as recorded in complementation studies with the atf1 gene, and a partial complementation of the osmotic and oxidative-stress-sensitive phenotypes was also achieved in submerged cultures. AtfA is therefore a true functional ortholog of fission yeast's Atf1. As demonstrated by RT-PCR experiments, elements of both oxidative (e.g. catalase B) and osmotic (e.g. glycerol-3-phosphate dehydrogenase B) stress defense systems were transcriptionally regulated by AtfA in a stress-type-specific manner. Deletion of atfA resulted in oxidative-stress-sensitive phenotypes while the high-osmolarity stress sensitivity of the fungus was not affected significantly. In A. nidulans, the glutathione/glutathione disulfide redox status of the cells as well as apoptotic cell death and autolysis seemed to be controlled by regulatory elements other than AtfA. In conclusion, the orchestrations of stress responses in the aspergilli and in fission yeast share several common features, but further studies are needed to answer the important question of whether a fission yeast-like core environmental stress response also operates in the euascomycete genus Aspergillus.",
keywords = "Apoptosis, Autolysis, Auxotrophy, CESR, Osmotic stress, Oxidative stress, Stress signaling",
author = "Anita Bal{\'a}zs and Imre P{\'o}csi and Z. Hamari and E. Leiter and T. Emri and M{\'a}rton Miskei and Judit Ol{\'a}h and Vikt{\'o}ria T{\'o}th and Nikoletta Hegedus and Prade, {Rolf A.} and M. Moln{\'a}r and I. P{\'o}csi",
year = "2010",
month = "3",
doi = "10.1007/s00438-010-0513-z",
language = "English",
volume = "283",
pages = "289--303",
journal = "Molecular Genetics and Genomics",
issn = "1617-4615",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - AtfA bZIP-type transcription factor regulates oxidative and osmotic stress responses in Aspergillus nidulans

AU - Balázs, Anita

AU - Pócsi, Imre

AU - Hamari, Z.

AU - Leiter, E.

AU - Emri, T.

AU - Miskei, Márton

AU - Oláh, Judit

AU - Tóth, Viktória

AU - Hegedus, Nikoletta

AU - Prade, Rolf A.

AU - Molnár, M.

AU - Pócsi, I.

PY - 2010/3

Y1 - 2010/3

N2 - The aim of the study was to demonstrate that the bZIP-type transcription factor AtfA regulates different types of stress responses in Aspergillus nidulans similarly to Atf1, the orthologous 'all-purpose' transcription factor of Schizosaccharomyces pombe. Heterologous expression of atfA in a S. pombe Δatf1 mutant restored the osmotic stress tolerance of fission yeast in surface cultures to the same level as recorded in complementation studies with the atf1 gene, and a partial complementation of the osmotic and oxidative-stress-sensitive phenotypes was also achieved in submerged cultures. AtfA is therefore a true functional ortholog of fission yeast's Atf1. As demonstrated by RT-PCR experiments, elements of both oxidative (e.g. catalase B) and osmotic (e.g. glycerol-3-phosphate dehydrogenase B) stress defense systems were transcriptionally regulated by AtfA in a stress-type-specific manner. Deletion of atfA resulted in oxidative-stress-sensitive phenotypes while the high-osmolarity stress sensitivity of the fungus was not affected significantly. In A. nidulans, the glutathione/glutathione disulfide redox status of the cells as well as apoptotic cell death and autolysis seemed to be controlled by regulatory elements other than AtfA. In conclusion, the orchestrations of stress responses in the aspergilli and in fission yeast share several common features, but further studies are needed to answer the important question of whether a fission yeast-like core environmental stress response also operates in the euascomycete genus Aspergillus.

AB - The aim of the study was to demonstrate that the bZIP-type transcription factor AtfA regulates different types of stress responses in Aspergillus nidulans similarly to Atf1, the orthologous 'all-purpose' transcription factor of Schizosaccharomyces pombe. Heterologous expression of atfA in a S. pombe Δatf1 mutant restored the osmotic stress tolerance of fission yeast in surface cultures to the same level as recorded in complementation studies with the atf1 gene, and a partial complementation of the osmotic and oxidative-stress-sensitive phenotypes was also achieved in submerged cultures. AtfA is therefore a true functional ortholog of fission yeast's Atf1. As demonstrated by RT-PCR experiments, elements of both oxidative (e.g. catalase B) and osmotic (e.g. glycerol-3-phosphate dehydrogenase B) stress defense systems were transcriptionally regulated by AtfA in a stress-type-specific manner. Deletion of atfA resulted in oxidative-stress-sensitive phenotypes while the high-osmolarity stress sensitivity of the fungus was not affected significantly. In A. nidulans, the glutathione/glutathione disulfide redox status of the cells as well as apoptotic cell death and autolysis seemed to be controlled by regulatory elements other than AtfA. In conclusion, the orchestrations of stress responses in the aspergilli and in fission yeast share several common features, but further studies are needed to answer the important question of whether a fission yeast-like core environmental stress response also operates in the euascomycete genus Aspergillus.

KW - Apoptosis

KW - Autolysis

KW - Auxotrophy

KW - CESR

KW - Osmotic stress

KW - Oxidative stress

KW - Stress signaling

UR - http://www.scopus.com/inward/record.url?scp=77949263551&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77949263551&partnerID=8YFLogxK

U2 - 10.1007/s00438-010-0513-z

DO - 10.1007/s00438-010-0513-z

M3 - Article

C2 - 20131067

AN - SCOPUS:77949263551

VL - 283

SP - 289

EP - 303

JO - Molecular Genetics and Genomics

JF - Molecular Genetics and Genomics

SN - 1617-4615

IS - 3

ER -