AtCRK5 protein kinase exhibits a regulatory role in hypocotyl hook development during skotomorphogenesis

Abu Imran Baba, Norbert Andrási, Ildikó Valkai, Teréz Gorcsa, Lilla Koczka, Zsuzsanna Darula, Katalin F. Medzihradszky, László Szabados, Attila Fehér, Gábor Rigó, Ágnes Cséplő

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Seedling establishment following germination requires the fine tuning of plant hormone levels including that of auxin. Directional movement of auxin has a central role in the associated processes, among others, in hypocotyl hook development. Regulated auxin transport is ensured by several transporters (PINs, AUX1, ABCB) and their tight cooperation. Here we describe the regulatory role of the Arabidopsis thaliana CRK5 protein kinase during hypocotyl hook formation/opening influencing auxin transport and the auxin-ethylene-GA hormonal crosstalk. It was found that the Atcrk5-1 mutant exhibits an impaired hypocotyl hook establishment phenotype resulting only in limited bending in the dark. The Atcrk5-1 mutant proved to be deficient in the maintenance of local auxin accumulation at the concave side of the hypocotyl hook as demonstrated by decreased fluorescence of the auxin sensor DR5::GFP. Abundance of the polar auxin transport (PAT) proteins PIN3, PIN7, and AUX1 were also decreased in the Atcrk5-1 hypocotyl hook. The AtCRK5 protein kinase was reported to regulate PIN2 protein activity by phosphorylation during the root gravitropic response. Here it is shown that AtCRK5 can also phosphorylate in vitro the hydrophilic loops of PIN3. We propose that AtCRK5 may regulate hypocotyl hook formation in Arabidopsis thaliana through the phosphorylation of polar auxin transport (PAT) proteins, the fine tuning of auxin transport, and consequently the coordination of auxin-ethylene-GA levels.

Original languageEnglish
Article number3432
JournalInternational journal of molecular sciences
Volume20
Issue number14
DOIs
Publication statusPublished - Jul 2 2019

Keywords

  • Arabidopsis thaliana
  • Auxin gradient
  • Ca/calmodulin-dependent kinase-related kinases (CRKs)
  • Ethylene
  • GA
  • Polar auxin transport (PAT) proteins
  • Skotomorphogenesis

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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