Astrocytes from mouse brain slices express ClC-2-mediated Cl- currents regulated during development and after injury

Judit K. Makara, Angelika Rappert, Katja Matthias, Christian Steinhäuser, A. Spät, Helmut Kettenmann

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Chloride channels are important for astrocytic volume regulation and K + buffering. We demonstrate functional expression of a hyperpolarization-activated Cl- current in a subpopulation of astrocytes in acute slices or after fresh isolation from adult brain of GFAP/EGFP transgenic animals in which astrocytes are selectively labeled. When Na+ and K+ were substituted with NMDG+ and Cs+ in extra- and intracellular solutions, an inward current was observed at negative membrane potentials. The current displayed features as described for a Cl- current characterized in cultured astrocytes: it activated time dependently at potentials negative to -40 mV, displayed no inactivation within 1 s, and was inhibited reversibly by submicromolar concentrations of Cd2+. The current was not detectable in astrocytes from ClC-2 knockout mice, indicating that the ClC-2 chloride channel generated the conductance. Current density was significantly lower in a corresponding population of astrocytes isolated from immature brain and in reactive astrocytes within a lesion site.

Original languageEnglish
Pages (from-to)521-530
Number of pages10
JournalMolecular and Cellular Neuroscience
Volume23
Issue number4
DOIs
Publication statusPublished - Aug 1 2003

Fingerprint

Astrocytes
Wounds and Injuries
Brain
Chloride Channels
Genetically Modified Animals
Knockout Mice
Membrane Potentials
Population

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Astrocytes from mouse brain slices express ClC-2-mediated Cl- currents regulated during development and after injury. / Makara, Judit K.; Rappert, Angelika; Matthias, Katja; Steinhäuser, Christian; Spät, A.; Kettenmann, Helmut.

In: Molecular and Cellular Neuroscience, Vol. 23, No. 4, 01.08.2003, p. 521-530.

Research output: Contribution to journalArticle

Makara, Judit K. ; Rappert, Angelika ; Matthias, Katja ; Steinhäuser, Christian ; Spät, A. ; Kettenmann, Helmut. / Astrocytes from mouse brain slices express ClC-2-mediated Cl- currents regulated during development and after injury. In: Molecular and Cellular Neuroscience. 2003 ; Vol. 23, No. 4. pp. 521-530.
@article{5a936a6acb2f4148bfdfe46cb04e0d03,
title = "Astrocytes from mouse brain slices express ClC-2-mediated Cl- currents regulated during development and after injury",
abstract = "Chloride channels are important for astrocytic volume regulation and K + buffering. We demonstrate functional expression of a hyperpolarization-activated Cl- current in a subpopulation of astrocytes in acute slices or after fresh isolation from adult brain of GFAP/EGFP transgenic animals in which astrocytes are selectively labeled. When Na+ and K+ were substituted with NMDG+ and Cs+ in extra- and intracellular solutions, an inward current was observed at negative membrane potentials. The current displayed features as described for a Cl- current characterized in cultured astrocytes: it activated time dependently at potentials negative to -40 mV, displayed no inactivation within 1 s, and was inhibited reversibly by submicromolar concentrations of Cd2+. The current was not detectable in astrocytes from ClC-2 knockout mice, indicating that the ClC-2 chloride channel generated the conductance. Current density was significantly lower in a corresponding population of astrocytes isolated from immature brain and in reactive astrocytes within a lesion site.",
author = "Makara, {Judit K.} and Angelika Rappert and Katja Matthias and Christian Steinh{\"a}user and A. Sp{\"a}t and Helmut Kettenmann",
year = "2003",
month = "8",
day = "1",
doi = "10.1016/S1044-7431(03)00080-0",
language = "English",
volume = "23",
pages = "521--530",
journal = "Molecular and Cellular Neurosciences",
issn = "1044-7431",
publisher = "Academic Press Inc.",
number = "4",

}

TY - JOUR

T1 - Astrocytes from mouse brain slices express ClC-2-mediated Cl- currents regulated during development and after injury

AU - Makara, Judit K.

AU - Rappert, Angelika

AU - Matthias, Katja

AU - Steinhäuser, Christian

AU - Spät, A.

AU - Kettenmann, Helmut

PY - 2003/8/1

Y1 - 2003/8/1

N2 - Chloride channels are important for astrocytic volume regulation and K + buffering. We demonstrate functional expression of a hyperpolarization-activated Cl- current in a subpopulation of astrocytes in acute slices or after fresh isolation from adult brain of GFAP/EGFP transgenic animals in which astrocytes are selectively labeled. When Na+ and K+ were substituted with NMDG+ and Cs+ in extra- and intracellular solutions, an inward current was observed at negative membrane potentials. The current displayed features as described for a Cl- current characterized in cultured astrocytes: it activated time dependently at potentials negative to -40 mV, displayed no inactivation within 1 s, and was inhibited reversibly by submicromolar concentrations of Cd2+. The current was not detectable in astrocytes from ClC-2 knockout mice, indicating that the ClC-2 chloride channel generated the conductance. Current density was significantly lower in a corresponding population of astrocytes isolated from immature brain and in reactive astrocytes within a lesion site.

AB - Chloride channels are important for astrocytic volume regulation and K + buffering. We demonstrate functional expression of a hyperpolarization-activated Cl- current in a subpopulation of astrocytes in acute slices or after fresh isolation from adult brain of GFAP/EGFP transgenic animals in which astrocytes are selectively labeled. When Na+ and K+ were substituted with NMDG+ and Cs+ in extra- and intracellular solutions, an inward current was observed at negative membrane potentials. The current displayed features as described for a Cl- current characterized in cultured astrocytes: it activated time dependently at potentials negative to -40 mV, displayed no inactivation within 1 s, and was inhibited reversibly by submicromolar concentrations of Cd2+. The current was not detectable in astrocytes from ClC-2 knockout mice, indicating that the ClC-2 chloride channel generated the conductance. Current density was significantly lower in a corresponding population of astrocytes isolated from immature brain and in reactive astrocytes within a lesion site.

UR - http://www.scopus.com/inward/record.url?scp=0042160243&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0042160243&partnerID=8YFLogxK

U2 - 10.1016/S1044-7431(03)00080-0

DO - 10.1016/S1044-7431(03)00080-0

M3 - Article

VL - 23

SP - 521

EP - 530

JO - Molecular and Cellular Neurosciences

JF - Molecular and Cellular Neurosciences

SN - 1044-7431

IS - 4

ER -