Astrocytes and neurons express the tight junction-specific protein occludin in vitro

H. Bauer, W. Stelzhammer, R. Fuchs, T. M. Weiger, C. Danninger, G. Probst, I. A. Krizbai

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Abstract

The expression of occludin, an integral plasma membrane protein specifically located at tight junctions, was studied in various epithelial and nonepithelial tissues by means of RT-PCR, Western blotting, and immunofluorescent staining. Besides detection in epithelial and endothelial tissue, expression of occludin was found in primary and secondary cultures of neurons and astrocytes. Differentiation of astrocytes in vitro led to a marked decrease in occludin expression. Extractability of occludin from plasma membranes differed considerably between epithelial and nonepithelial cells. Following treatment with Triton X-100, occludin was completely extracted from astrocytic membranes but not from membranes derived from MDCK cells, suggesting a difference in the cytoplasmic and/or plasma membrane anchoring of occludin between these cell types.

Original languageEnglish
Pages (from-to)434-438
Number of pages5
JournalExperimental Cell Research
Volume250
Issue number2
DOIs
Publication statusPublished - Aug 1 1999

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Keywords

  • Astrocytes
  • Cell culture
  • Confocal laser microscopy
  • Neurons
  • Occludin
  • RT-PCR
  • Tight junctions
  • Western blotting

ASJC Scopus subject areas

  • Cell Biology

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