Association of vinculin to the platelet cytoskeleton during thrombin-induced aggregation

Guus M. Asyee, Auguste Sturk, László Muszbek

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Vinculin is a protein generally believed to be involved in membrane-cytoskeleton interaction, and its presence in platelets has been verified earlier. Here we show that in resting bovine platelets, vinculin is not associated with the Triton-insoluble cytoskeletal fraction but becomes incorporated into it during the thrombin-induced activation process. The incorporation starts around the same time as the release reaction and only after the shape change and the first phase of aggregation have taken place. Its time course parallels the cytoskeletal association of actin and certain other contractile proteins. Vinculin is a minor component of platelet cytoskeleton and only about 10 % of the total platelet vinculin becomes incorporated into the Triton X-100 residue.

Original languageEnglish
Pages (from-to)358-364
Number of pages7
JournalExperimental Cell Research
Volume168
Issue number2
DOIs
Publication statusPublished - Feb 1987

ASJC Scopus subject areas

  • Cell Biology

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