Association of TLR-4 and TNF-alpha polymorphisms with clinical symptoms and cytokine levels in patients with allergic rhinitis

Magdolna Krasznai, Kinga Szaniszlo, Helga Kraxner, Eszter Vargha, M. Kovács, Zoltan Borocz, Edit Kaszas, Gyorgyi Mezei, Endre Cserhati, K. Cseh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Our objective was to determine the frequency of TNF-alpha -238, -308 G/A promoter and TLR-4 299 D/G and 399 T/I polymorphisms in healthy population and in patients with seasonal allergic rhinitis, and to examine its influences on serum TNF-alpha, TNF receptor-1, Fas, Fas-ligand, IgE levels and on clinical symptoms. A pilot study was performed in 66 patients with seasonal allergic rhinitis to ragweed pollen and 161 non-allergic subjects using PCR-RFLP technique and ELISA. Carriers of the -238A and -308G alleles have significantly higher TNF-alpha and IgE levels, clinical score values and lower peak nasal flow (PNIF) values during and after ragweed pollen season. Patients with the 299G/399I alleles of the TLR-4 gene have significantly lower TNF-alpha, Fas, FasL and IgE levels, clinical scores and higher PNIF values during and after pollen season. The -238A and -308G polymorphisms of the TNF-alpha promoter and 299D/399T polymorphisms of the TLR-4 gene are associated with more pronounced clinical symptoms, higher cytokine and IgE levels, and low PNIF values. These polymorphisms are very likely to contribute to the heterogeneity of clinical and laboratory parameters of patients.

Original languageEnglish
Pages (from-to)561-567
Number of pages7
JournalEuropean Archives of Oto-Rhino-Laryngology
Volume268
Issue number4
DOIs
Publication statusPublished - Apr 2011

Fingerprint

Tumor Necrosis Factor-alpha
Immunoglobulin E
Cytokines
Seasonal Allergic Rhinitis
Alleles
Fas Ligand Protein
Tumor Necrosis Factor Receptors
Pollen
Nose
Restriction Fragment Length Polymorphisms
Genes
Enzyme-Linked Immunosorbent Assay
Allergic Rhinitis
Polymerase Chain Reaction
Serum
Population
ragweed pollen

Keywords

  • Allergic rhinitis
  • Fas
  • FasL
  • IgE
  • TLR-4
  • TNF-alpha

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Association of TLR-4 and TNF-alpha polymorphisms with clinical symptoms and cytokine levels in patients with allergic rhinitis. / Krasznai, Magdolna; Szaniszlo, Kinga; Kraxner, Helga; Vargha, Eszter; Kovács, M.; Borocz, Zoltan; Kaszas, Edit; Mezei, Gyorgyi; Cserhati, Endre; Cseh, K.

In: European Archives of Oto-Rhino-Laryngology, Vol. 268, No. 4, 04.2011, p. 561-567.

Research output: Contribution to journalArticle

Krasznai, Magdolna ; Szaniszlo, Kinga ; Kraxner, Helga ; Vargha, Eszter ; Kovács, M. ; Borocz, Zoltan ; Kaszas, Edit ; Mezei, Gyorgyi ; Cserhati, Endre ; Cseh, K. / Association of TLR-4 and TNF-alpha polymorphisms with clinical symptoms and cytokine levels in patients with allergic rhinitis. In: European Archives of Oto-Rhino-Laryngology. 2011 ; Vol. 268, No. 4. pp. 561-567.
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