Association of thrombin, plasmin, thrombin-antithrombin III complex and plasmin-antithrombin III complex with isolated hepatocytes

Zoltán Spolarics, Miklós P. Kalapos, István Léránt, Tamás Garzó, Ferene Antoni, József Mandl, Raymund Machovich

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The interaction of thrombin, plasmin on their antithrombin III complexes with isolated mouse hepatocytes was studied. Plasmin bound to hepatocytes in a concentration-dependent manner with an apparent Kd of 6.4 · 10-8 M, attaining equilibrium within 10 min, and the interaction was inhibited by 6-amino-n-hexanoic acid. Plasmin treated with diisopropylfluorophosphate (DFP) bound to the cells in similar way as the untreated form of the enzyme. Thrombin bound also to hepatocytes, in a concentration-dependent manner, with a Kd of 5.4 · 10-8 M reaching a steady state after 180 min. Thrombin inactivated with DFP, however, was inhibited in its binding to these cells. These data suggest that, whereas the kringle domains of plasmin are responsible for the enzyme-cell interaction, the active center of thrombin may be involved in the binding of this enzyme to hepatocytes. Plasmin-antithrombin III and thrombin-antithrombin III complexes were also associated with hepatocytes in a time-dependent manner, reaching a plateau after 180 min, and the two complexes competed in the interaction. While the interaction of active proteinases plasmin or thrombin with hepatocytes did not result in their internalization, the antithrombin III complexes were taken up by the cells, and thrombin-antithrombin III complex was degraded. These results indicate that hepatocytes may participate in the elimination of proteinase-antithrombin III complexes from the plasma, while the association of plasmin and thrombin with hepatocytes could imply distinct biological importance.

Original languageEnglish
Pages (from-to)231-236
Number of pages6
JournalBBA - Molecular Cell Research
Volume1012
Issue number3
DOIs
Publication statusPublished - Aug 15 1989

Keywords

  • (Mouse hepatocyte)
  • Antithrombin III
  • Plasmin
  • Protein degradation
  • Thrombin

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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