Association of the AMPA receptor-related postsynaptic density proteins GRIP and ABP with subsets of glutamate-sensitive neurons in the rat retina

R. Gábriel, Sunita De Souza, Edward B. Ziff, Paul Witkovsky

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We used specific antibodies against two postsynaptic density proteins, GRIP (glutamate receptor interacting protein) and ABP (AMPA receptor-binding protein), to study their distribution in the rat retina. In the central nervous system, it has been shown that both proteins bind strongly to the AMPA glutamate receptor (GluR) 2/3 subunits, but not other GluRs, through a set of three PDZ domains. Western blots detected a single GRIP protein that was virtually identical in retina and brain, whereas retinal ABP corresponded to only one of three ABP peptides found in brain. The retinal distributions of GluR2/3, GRIP, and ABP immunoreactivity (IR) were similar but not identical. GluR2/3 immunoreactivity (IR) was abundant in both plexiform layers and in large perikarya. ABP IR was concentrated in large perikarya but was sparse in the plexiform layers, whereas GRIP IR was relatively more abundant in the plexiform layers than in perikarya. Immunolabel for these three antibodies consisted of puncta ≤ 0.2 μm in diameter. The cellular localization of GRIP and ABP IR was examined by double labeling subclasses of retinal neuron with characteristic marker proteins, e.g., calbindin. GRIP, ABP, and GluR2/3 IR were detected in horizontal cells, dopaminergic and glycinergic AII amacrine cells and large ganglion cells. Immunolabel was absent in rod bipolar and weak or absent in cholinergic amacrine cells. By using the tyramide method of signal amplification, a colocalization of GluR2/3 was found with either GRIP or ABP in horizontal cell terminals, and perikarya of amacrine and ganglion cells. Our results show that ABP and GRIP colocalize with GluR2/3 in particular subsets of retinal neuron, as was previously established for certain neurons in the brain.

Original languageEnglish
Pages (from-to)129-140
Number of pages12
JournalJournal of Comparative Neurology
Volume449
Issue number2
DOIs
Publication statusPublished - Jul 22 2002

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Receptor-Interacting Protein Serine-Threonine Kinases
AMPA Receptors
Glutamate Receptors
Retina
Glutamic Acid
Carrier Proteins
Neurons
Amacrine Cells
Retinal Neurons
Ganglia
Brain
postsynaptic density proteins
PDZ Domains
Calbindins
Proteins
Antibodies
Cholinergic Agents
Central Nervous System
Western Blotting

Keywords

  • Amacrine cell
  • Ganglion cell
  • Glutamate receptor
  • Horizontal cell
  • Immunocytochemistry

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Association of the AMPA receptor-related postsynaptic density proteins GRIP and ABP with subsets of glutamate-sensitive neurons in the rat retina. / Gábriel, R.; De Souza, Sunita; Ziff, Edward B.; Witkovsky, Paul.

In: Journal of Comparative Neurology, Vol. 449, No. 2, 22.07.2002, p. 129-140.

Research output: Contribution to journalArticle

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