Association of protein kinase-C-α with cytoplasmic vesicles in melanoma cells

J. Tímár, Bin Liu, Rajesh Bazaz, Kenneth V. Honn

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In B16a melanoma cells, protein kinase-C-α (PKCα) is immunomorphologically associated with cytoplasmic vesicles in addition to the previously observed locations (plasma membrane, cytoskeleton, nucleus), as detected with monoclonal antibody (MAb) MC3a. Subcellular fractionation indicated that the authentic 80-KD protein as well as PKC activity can be detected in several particulate fractions except for L2, which contains dense lysosomes. The highest PKC activity is associated with the cytosol- ultralight vesicles and the L1 fraction (containing plasma membrane, endosomes, and the Golgi apparatus). Both of these fractions contained the fluid-phase endocytosis marker peroxidase, indicating that PKCα, in addition to other subcellular structures, is most probably associated with endosomal membranes in B16a melanoma cells.

Original languageEnglish
Pages (from-to)177-182
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Volume44
Issue number2
Publication statusPublished - Feb 1996

Fingerprint

Cytoplasmic Vesicles
Protein Kinase C
Melanoma
Cell Membrane
Endosomes
Golgi Apparatus
Endocytosis
Lysosomes
Cytoskeleton
Cytosol
Peroxidase
Monoclonal Antibodies
Membranes
Proteins

Keywords

  • Endosomes
  • Immunofluorescence
  • Melanoma
  • PKCα
  • Vesicles

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Association of protein kinase-C-α with cytoplasmic vesicles in melanoma cells. / Tímár, J.; Liu, Bin; Bazaz, Rajesh; Honn, Kenneth V.

In: Journal of Histochemistry and Cytochemistry, Vol. 44, No. 2, 02.1996, p. 177-182.

Research output: Contribution to journalArticle

Tímár, J. ; Liu, Bin ; Bazaz, Rajesh ; Honn, Kenneth V. / Association of protein kinase-C-α with cytoplasmic vesicles in melanoma cells. In: Journal of Histochemistry and Cytochemistry. 1996 ; Vol. 44, No. 2. pp. 177-182.
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