Association of ARTS1 gene polymorphisms with ankylosing spondylitis in the Hungarian population: The rs27044 variant is associated with HLA-B*2705 subtype in Hungarian patients with ankylosing spondylitis

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Abstract

Objective. Associations have been found between ankylosing spondylitis (AS) and polymorphisms in the aminopeptidase regulator of TNFR1 shedding (ARTS1) gene. We studied the association of 5 polymorphisms within the ARTS1 gene with AS in Hungarian patients. We also investigated the prevalence of HLA-B27 subtypes in the Hungarian population. Methods. A case-control study including 297 patients with AS and 200 sex and ethnically matched healthy controls was performed. Patients and controls were genotyped for rs27044, rs17482078, rs10050860, rs30187, and rs2287987 single-nucleotide polymorphisms using real-time polymerase chain reaction (PCR) allelic discrimination. HLA-B27 subtypes were determined with PCR sequence-specific primer (PCR-SSP) technique. Results. We observed a significant increase in the minor allele frequency of rs27044 (p = 0.001) in the AS group compared to controls. The minor allele frequencies of rs10050860 (p = 0.006) and rs2287987 (p = 0.002) showed a significant decrease inAS patients compared to controls. Haplotype analysis revealed association of 2 ARTS1 haplotypes with AS in the Hungarian population. We found that HLA-B*2705 was the predominant subtype in Hungarians with AS. Carriage of the G allele of rs27044 was significantly associated with the HLA-B*2705 subtype (p = 0.009) in AS patients. Conclusion. We confirmed reported associations of ARTS1 gene polymorphisms with AS in a Hungarian cohort study. We found HLA-B*2705 as the predominant subtype in Hungarian AS patients in accord with other studies on Caucasian populations. Our results suggest that the ARTS1 gene variants together with HLA-B27 strongly contribute to disease susceptibility in patients with AS. The Journal of Rheumatology

Original languageEnglish
Pages (from-to)379-384
Number of pages6
JournalJournal of Rheumatology
Volume37
Issue number2
DOIs
Publication statusPublished - Feb 1 2010

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Keywords

  • Ankylosing spondylitis
  • Arts1
  • HLA-B27
  • Polymorphism

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology

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