Association between allelic polymorphisms of metabolizing enzymes (CYP 1A1, CYP 1A2, CYP 2E1, mEH) and occurrence of colorectal cancer in Hungary

István Kiss, Zsuzsa Orsós, Katalin Gombos, Barna Bogner, András Csejtei, Antal Tibold, Zsuzsa Varga, Emese Pázsit, Ingrid Magda, Annamária Zólyomi, I. Ember

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Background: Genetic polymorphisms of metabolizing enzymes may affect the risk of cancer formation in humans. Since the diet can contain polycyclic aromatic hydrocarbons (PAHs) and heterocyclic amines (HAs), the relationship between polymorphisms of enzymes involved in PAH and HA metabolism and the occurrence of sporadic colorectal cancer was studied. Patients and Methods: Five hundred colorectal cancer patients and 500 controls were genotyped for cytochrome P450 enzymes (CYP) 1A1 Ile/Val, CYP 1A2*1F, CYP 2E1 c1/c2, microsomal epoxy hydrolase (mEH) exon 3 Tyr113His and exon 4 His139Arg polymorphisms by allele-specific polymerase chain reaction (PCR) or PCR-restriction fragmenth length polymorphism (RFLP). Results: The presence of CYP 1A1 Val, CYP 2E1 c2 and mEH exon 3 His alleles was statistically significantly associated with the occurrence of colorectal cancer (OR: 1.44 95% CI: 1.04-2.00; OR: 1.74 95% CI: 1.15-2.65; OR: 1.79 95% CI: 1.10-2.92, respectively). Conclusion: These findings suggest that allelic polymorphism of metabolizing enzymes play an important role in human colorectal carcinogenesis by affecting the metabolism of dietary carcinogens.

Original languageEnglish
Pages (from-to)2931-2937
Number of pages7
JournalAnticancer Research
Volume27
Issue number4 C
Publication statusPublished - Jul 2007

Fingerprint

Cytochrome P-450 CYP2E1
Cytochrome P-450 CYP1A2
Hungary
Hydrolases
Cytochrome P-450 Enzyme System
Colorectal Neoplasms
Enzymes
Exons
Polycyclic Aromatic Hydrocarbons
Amines
Alleles
Polymerase Chain Reaction
Genetic Polymorphisms
Carcinogens
Carcinogenesis
Diet

Keywords

  • Colorectal cancer
  • CYP 1A1
  • CYP 1A2
  • CYP 2E1
  • Genetic polymorphism
  • mEH

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Association between allelic polymorphisms of metabolizing enzymes (CYP 1A1, CYP 1A2, CYP 2E1, mEH) and occurrence of colorectal cancer in Hungary. / Kiss, István; Orsós, Zsuzsa; Gombos, Katalin; Bogner, Barna; Csejtei, András; Tibold, Antal; Varga, Zsuzsa; Pázsit, Emese; Magda, Ingrid; Zólyomi, Annamária; Ember, I.

In: Anticancer Research, Vol. 27, No. 4 C, 07.2007, p. 2931-2937.

Research output: Contribution to journalArticle

Kiss, I, Orsós, Z, Gombos, K, Bogner, B, Csejtei, A, Tibold, A, Varga, Z, Pázsit, E, Magda, I, Zólyomi, A & Ember, I 2007, 'Association between allelic polymorphisms of metabolizing enzymes (CYP 1A1, CYP 1A2, CYP 2E1, mEH) and occurrence of colorectal cancer in Hungary', Anticancer Research, vol. 27, no. 4 C, pp. 2931-2937.
Kiss, István ; Orsós, Zsuzsa ; Gombos, Katalin ; Bogner, Barna ; Csejtei, András ; Tibold, Antal ; Varga, Zsuzsa ; Pázsit, Emese ; Magda, Ingrid ; Zólyomi, Annamária ; Ember, I. / Association between allelic polymorphisms of metabolizing enzymes (CYP 1A1, CYP 1A2, CYP 2E1, mEH) and occurrence of colorectal cancer in Hungary. In: Anticancer Research. 2007 ; Vol. 27, No. 4 C. pp. 2931-2937.
@article{1a98d4418062495fa4f9670bdc5ac6d6,
title = "Association between allelic polymorphisms of metabolizing enzymes (CYP 1A1, CYP 1A2, CYP 2E1, mEH) and occurrence of colorectal cancer in Hungary",
abstract = "Background: Genetic polymorphisms of metabolizing enzymes may affect the risk of cancer formation in humans. Since the diet can contain polycyclic aromatic hydrocarbons (PAHs) and heterocyclic amines (HAs), the relationship between polymorphisms of enzymes involved in PAH and HA metabolism and the occurrence of sporadic colorectal cancer was studied. Patients and Methods: Five hundred colorectal cancer patients and 500 controls were genotyped for cytochrome P450 enzymes (CYP) 1A1 Ile/Val, CYP 1A2*1F, CYP 2E1 c1/c2, microsomal epoxy hydrolase (mEH) exon 3 Tyr113His and exon 4 His139Arg polymorphisms by allele-specific polymerase chain reaction (PCR) or PCR-restriction fragmenth length polymorphism (RFLP). Results: The presence of CYP 1A1 Val, CYP 2E1 c2 and mEH exon 3 His alleles was statistically significantly associated with the occurrence of colorectal cancer (OR: 1.44 95{\%} CI: 1.04-2.00; OR: 1.74 95{\%} CI: 1.15-2.65; OR: 1.79 95{\%} CI: 1.10-2.92, respectively). Conclusion: These findings suggest that allelic polymorphism of metabolizing enzymes play an important role in human colorectal carcinogenesis by affecting the metabolism of dietary carcinogens.",
keywords = "Colorectal cancer, CYP 1A1, CYP 1A2, CYP 2E1, Genetic polymorphism, mEH",
author = "Istv{\'a}n Kiss and Zsuzsa Ors{\'o}s and Katalin Gombos and Barna Bogner and Andr{\'a}s Csejtei and Antal Tibold and Zsuzsa Varga and Emese P{\'a}zsit and Ingrid Magda and Annam{\'a}ria Z{\'o}lyomi and I. Ember",
year = "2007",
month = "7",
language = "English",
volume = "27",
pages = "2931--2937",
journal = "Anticancer Research",
issn = "0250-7005",
publisher = "International Institute of Anticancer Research",
number = "4 C",

}

TY - JOUR

T1 - Association between allelic polymorphisms of metabolizing enzymes (CYP 1A1, CYP 1A2, CYP 2E1, mEH) and occurrence of colorectal cancer in Hungary

AU - Kiss, István

AU - Orsós, Zsuzsa

AU - Gombos, Katalin

AU - Bogner, Barna

AU - Csejtei, András

AU - Tibold, Antal

AU - Varga, Zsuzsa

AU - Pázsit, Emese

AU - Magda, Ingrid

AU - Zólyomi, Annamária

AU - Ember, I.

PY - 2007/7

Y1 - 2007/7

N2 - Background: Genetic polymorphisms of metabolizing enzymes may affect the risk of cancer formation in humans. Since the diet can contain polycyclic aromatic hydrocarbons (PAHs) and heterocyclic amines (HAs), the relationship between polymorphisms of enzymes involved in PAH and HA metabolism and the occurrence of sporadic colorectal cancer was studied. Patients and Methods: Five hundred colorectal cancer patients and 500 controls were genotyped for cytochrome P450 enzymes (CYP) 1A1 Ile/Val, CYP 1A2*1F, CYP 2E1 c1/c2, microsomal epoxy hydrolase (mEH) exon 3 Tyr113His and exon 4 His139Arg polymorphisms by allele-specific polymerase chain reaction (PCR) or PCR-restriction fragmenth length polymorphism (RFLP). Results: The presence of CYP 1A1 Val, CYP 2E1 c2 and mEH exon 3 His alleles was statistically significantly associated with the occurrence of colorectal cancer (OR: 1.44 95% CI: 1.04-2.00; OR: 1.74 95% CI: 1.15-2.65; OR: 1.79 95% CI: 1.10-2.92, respectively). Conclusion: These findings suggest that allelic polymorphism of metabolizing enzymes play an important role in human colorectal carcinogenesis by affecting the metabolism of dietary carcinogens.

AB - Background: Genetic polymorphisms of metabolizing enzymes may affect the risk of cancer formation in humans. Since the diet can contain polycyclic aromatic hydrocarbons (PAHs) and heterocyclic amines (HAs), the relationship between polymorphisms of enzymes involved in PAH and HA metabolism and the occurrence of sporadic colorectal cancer was studied. Patients and Methods: Five hundred colorectal cancer patients and 500 controls were genotyped for cytochrome P450 enzymes (CYP) 1A1 Ile/Val, CYP 1A2*1F, CYP 2E1 c1/c2, microsomal epoxy hydrolase (mEH) exon 3 Tyr113His and exon 4 His139Arg polymorphisms by allele-specific polymerase chain reaction (PCR) or PCR-restriction fragmenth length polymorphism (RFLP). Results: The presence of CYP 1A1 Val, CYP 2E1 c2 and mEH exon 3 His alleles was statistically significantly associated with the occurrence of colorectal cancer (OR: 1.44 95% CI: 1.04-2.00; OR: 1.74 95% CI: 1.15-2.65; OR: 1.79 95% CI: 1.10-2.92, respectively). Conclusion: These findings suggest that allelic polymorphism of metabolizing enzymes play an important role in human colorectal carcinogenesis by affecting the metabolism of dietary carcinogens.

KW - Colorectal cancer

KW - CYP 1A1

KW - CYP 1A2

KW - CYP 2E1

KW - Genetic polymorphism

KW - mEH

UR - http://www.scopus.com/inward/record.url?scp=34547769925&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34547769925&partnerID=8YFLogxK

M3 - Article

VL - 27

SP - 2931

EP - 2937

JO - Anticancer Research

JF - Anticancer Research

SN - 0250-7005

IS - 4 C

ER -