Assisted fertilization by subzonal insemination and intracytoplasmic sperm injection

Andre Van Steirteghem, Jiaen Liu, Hubert Joris, Zsolt Nagy, Catherine Staessen, Michel Camus, Arjoko Wisanto, Elvire Van Assche, Paul Devroey

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21 Citations (Scopus)


The results of 600 consecutive treatment cycles of subzonal insemination (SUZI) and intracytoplasmic sperm injection (ICSI) are described in couples with failed fertilization after standard IVF or insufficient spermatozoa in the ejaculate for IVF. More oocytes were damaged by ICSI (16-3%) than by SUZI (8-5%) and the normal fertilization rate was substantially higher after ICSI (49-1% v. 16-6%). Subsequent development of two-pronuclear oocytes in vitro was 80% after SUZI and 73-9% after ICSI. Significantly more triple embryo replacements were carried out after ICSI than after SUZI. Embryo transfers were possible in 421 of the 600 cycles. There were 63 pregnancies after ICSI (215 transfers) and 23 after SUZI (156 transfers); 10 additional pregnancies were achieved after 50 transfers of a mixture of SUZI and ICSI embryos. The results of fetal karyotypes and follow-up of the children do not indicate an increase in congenital malformations.

Original languageEnglish
Pages (from-to)85-91
Number of pages7
JournalReproduction, Fertility and Development
Issue number1
Publication statusPublished - Jan 1 1994



  • In vitro fertilization
  • Male factor infertility
  • Micromanipulation

ASJC Scopus subject areas

  • Biotechnology
  • Reproductive Medicine
  • Animal Science and Zoology
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Developmental Biology

Cite this

Steirteghem, A. V., Liu, J., Joris, H., Nagy, Z., Staessen, C., Camus, M., Wisanto, A., Van Assche, E., & Devroey, P. (1994). Assisted fertilization by subzonal insemination and intracytoplasmic sperm injection. Reproduction, Fertility and Development, 6(1), 85-91.