Assignment problems of amino acids, di- and tripeptides and proteins in the near infrared region

S. Holly, O. Egyed, G. Jalsovszky

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Abstract

Nine amino acids, four dipeptides, one tripeptide and several types of proteins were studied by reflectance and absorption FT-IR spectroscopy. Mid-IR and, where necessary, Raman frequencies were applied to assign the combination and overtone bands occurring in the near-IR region. On one occasion, an isotope effect (deuterization) was also employed for the recognition of the combinations involving N+H3 vibrations. The overtone and combination bands of N+H3, CH3, CH2, NH2 and amide groups were, in part, assigned. On the basis of this assignment and with the use of second derivative and/or Fourier deconvolved spectra, attempts were made to recognize the characteristic features of glutamine and asparagine components of some proteins. The IR spectra were taken on an FT-IR spectrometer with a configuration allowing spectra to be measured in the 10,000 to 400 cm-1 range.

Original languageEnglish
Pages (from-to)101-109
Number of pages9
JournalSpectrochimica Acta Part A: Molecular Spectroscopy
Volume48
Issue number1
DOIs
Publication statusPublished - 1992

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Amino acids
Infrared radiation
Proteins
Infrared spectrometers
Absorption spectroscopy
Amides
Isotopes
Infrared spectroscopy
Derivatives
Dipeptides

ASJC Scopus subject areas

  • Engineering(all)

Cite this

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abstract = "Nine amino acids, four dipeptides, one tripeptide and several types of proteins were studied by reflectance and absorption FT-IR spectroscopy. Mid-IR and, where necessary, Raman frequencies were applied to assign the combination and overtone bands occurring in the near-IR region. On one occasion, an isotope effect (deuterization) was also employed for the recognition of the combinations involving N+H3 vibrations. The overtone and combination bands of N+H3, CH3, CH2, NH2 and amide groups were, in part, assigned. On the basis of this assignment and with the use of second derivative and/or Fourier deconvolved spectra, attempts were made to recognize the characteristic features of glutamine and asparagine components of some proteins. The IR spectra were taken on an FT-IR spectrometer with a configuration allowing spectra to be measured in the 10,000 to 400 cm-1 range.",
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AB - Nine amino acids, four dipeptides, one tripeptide and several types of proteins were studied by reflectance and absorption FT-IR spectroscopy. Mid-IR and, where necessary, Raman frequencies were applied to assign the combination and overtone bands occurring in the near-IR region. On one occasion, an isotope effect (deuterization) was also employed for the recognition of the combinations involving N+H3 vibrations. The overtone and combination bands of N+H3, CH3, CH2, NH2 and amide groups were, in part, assigned. On the basis of this assignment and with the use of second derivative and/or Fourier deconvolved spectra, attempts were made to recognize the characteristic features of glutamine and asparagine components of some proteins. The IR spectra were taken on an FT-IR spectrometer with a configuration allowing spectra to be measured in the 10,000 to 400 cm-1 range.

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