Assessment of the metabolic activity of Acremonium chrysogenum using Acridine Orange

E. Sándor, L. Karaffa, Gopal C. Paul, I. Pócsi, Colin R. Thomas, A. Szentirmai

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A method is described for the assessment of the metabolic activity of the filamentous fungus Acremonium chrysogenum using the fluorescent dye Acridine Orange. Changes in metabolic activity are indicated by a reversible red-green shift in the colour of the dye, quantifiable by image analysis. A. chlysogenum mycelia exhibited an overwhelmingly green colour in circumstances leading to high substrate uptake, while under carbon starvation they appeared orange-red. It is believed these colour changes reflected changes in internal pH. The method provides a visual tool for the investigation of the metabolic behaviour of filamentous fungi.

Original languageEnglish
Pages (from-to)693-697
Number of pages5
JournalBiotechnology Letters
Volume22
Issue number8
DOIs
Publication statusPublished - 2000

Fingerprint

Acremonium
Acridine Orange
Color
Fungi
Dyes
Mycelium
Starvation
Fluorescent Dyes
Image analysis
Coloring Agents
Carbon
Substrates

Keywords

  • Acremonium chrysogenum
  • Acridine Orange
  • Metabolic activity

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Assessment of the metabolic activity of Acremonium chrysogenum using Acridine Orange. / Sándor, E.; Karaffa, L.; Paul, Gopal C.; Pócsi, I.; Thomas, Colin R.; Szentirmai, A.

In: Biotechnology Letters, Vol. 22, No. 8, 2000, p. 693-697.

Research output: Contribution to journalArticle

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