Assessment of the Effects of Various UV Sources on Inactivation and Photoproduct Induction in Phage T7 Dosimeter

A. Fekete, A. A. Vink, S. Gaspar, A. Berces, K. Modos, Gy Ronto, L. Roza

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

The correlation between the biologically effective dose (BED) of a phage T7 biological dosimeter and the induction of cyclobutane pyrimidine dimers (CPD) and (6-4) photoproducts ((6-4)PD) in the phage DNA was determined using seven various UV sources. The BED is the inactivation rate of phage T7 expressed in HT7 units. The CPD and (6-4)PD were determined by lesion-specific monoclonal antibodies in an immunodot-blot assay. The various lamps induced these lesions at different rates; the relative induction ratios of CPD to (6-4)PD increased with increasing effective wavelength of irradiation source. The amount of total adducts per phage was compared to the BED of phage T7 dosimeter, representing the average number of UV lesions in phage. For UVC (200-280 nm radiation) and unfiltered TL01 the number of total adducts approximates the reading; however, UV sources having longer effective wavelengths produced fewer CPD and (6-4)PD. A possible explanation is that although the most relevant lesions by UVC are the CPD and (6-4)PD, at longer wavelengths other photoproducts can contribute to the lethal damage of phages. The results emphasize the need to study the biological effects of solar radiation because the lesions responsible for the lethal effect may be different from those produced by various UV sources.

Original languageEnglish
Pages (from-to)527-531
Number of pages5
JournalPhotochemistry and Photobiology
Volume68
Issue number4
Publication statusPublished - Oct 1998

Fingerprint

Bacteriophage T7
Pyrimidine Dimers
cyclobutane
Bacteriophages
Dosimeters
pyrimidines
deactivation
lesions
dosimeters
induction
dimers
dosage
adducts
wavelengths
Wavelength
Dosimetry
biological effects
Radiation Effects
solar radiation
antibodies

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics

Cite this

Assessment of the Effects of Various UV Sources on Inactivation and Photoproduct Induction in Phage T7 Dosimeter. / Fekete, A.; Vink, A. A.; Gaspar, S.; Berces, A.; Modos, K.; Ronto, Gy; Roza, L.

In: Photochemistry and Photobiology, Vol. 68, No. 4, 10.1998, p. 527-531.

Research output: Contribution to journalArticle

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