Assembly of the photosynthetic apparatus in embryos from Fucus serratus l

Morgane Lamote, Eva Darko, Benoît Schoefs, Yves Lemoine

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9 Citations (Scopus)

Abstract

The assembly of the photosynthetic apparatus was studied during the first six days of development of Fucus serratus L. embryos. HPLC analysis revealed that oospheres and zygotes contain the same photosynthetic pigments (i.e., chlorophyll a, chlorophyll c, fucoxanthin, violaxanthin, and β-carotene) as fully developed thalli. Total pigment amount increased after fertilization, mainly due to an active synthesis of Chl a and fucoxanthin. Spectral modifications revealing the progressive integration of Chl a and Chl c in the photosynthetic units are described. In particular, a distinct emission at 705 nm, reflecting the accumulation of LHC I, was clearly detected. The emission bands at 705 nm and 725 nm were characterized by 77 K excitation fluorescence measurements. Their spectra differed by the presence of a large band at approximately 550 nm due to fucoxanthin in the excitation spectrum of F705 nm. Room temperature variable fluorescence was first observed 30 h after fertilization indicating a functional Photosystem II electron transfer at this developmental stage.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalPhotosynthesis research
Volume77
Issue number1
DOIs
Publication statusPublished - Sep 17 2003

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Keywords

  • Biogenesis of the photosynthetic apparatus
  • Embryonic tissues
  • Macrophytes
  • Photosynthesis
  • Pigment-protein complexes
  • Seaweeds
  • Steady-state fluorescence spectroscopy
  • Variable fluorescence

ASJC Scopus subject areas

  • Biochemistry
  • Plant Science
  • Cell Biology

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