Assay of oxidized fibrinogen reactivity (OFR) as a biomarker of oxidative stress in human plasma: The role of lysine analogs

László Selmeci, Leila Seres, Mária Székely, Pál Soós, György Acsády

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background: There is accumulating evidence that fibrinogen is also a biomarker of oxidative stress in human plasma. Results of in vitro studies demonstrated that fibrinogen can bind to apolipoprotein(a) [apo(a)] component of lipoprotein(a) [Lp(a)] through both lysine-sensitive and lysine-insensitive mechanisms. The goal of the present study was to investigate oxidized fibrinogen reactivity (OFR) as a biomarker of oxidative stress in human plasma in the presence and absence of lysine analogs. Methods: Citrate anticoagulated peripheral venous blood samples were collected from 65 (36 M/29 F) consecutive patients with various peripheral vascular diseases. After centrifugation, the plasma was used promptly. Plasma OFR was determined in duplicate using a recently described kinetic photometric assay (358 nm, 37°C) in the presence and in the absence of lysine analogs. Results: The inclusion of tranexemic acid (TRA) or ε-aminocaproic acid in the incubation medium resulted in a rapid increase in OFR in a dose-dependent manner. The peak effect was observed at a final concentration of 200 mmol/L TRA. OFR was significantly higher in patient plasma assayed in the presence of TRA compared with no TRA (163.1±73.5 vs. 63.4±20.7 U/L; p<0.0001). Bound OFR was also significantly higher than free OFR (99.7±56.3 vs. 63.4±20.7; p<0.001). Conclusions: On the basis of the present results it appears that oxidized fibrinogen resides in plasma in two compartments: free and bound to apo(a) of Lp(a). The relatively simple and cost-effective kinetic approach applied in this study makes routine determination of OFR available as a biomarker of oxidative stress, separately in both compartments.

Original languageEnglish
Pages (from-to)379-382
Number of pages4
JournalClinical Chemistry and Laboratory Medicine
Volume48
Issue number3
DOIs
Publication statusPublished - Mar 1 2010

Keywords

  • Apolipoprotein(a)-bound fibrinogen
  • Epsilonaminocaproic acid
  • Oxidative stress
  • Oxidized fibrinogen reactivity
  • Tranexemic acid

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

Fingerprint Dive into the research topics of 'Assay of oxidized fibrinogen reactivity (OFR) as a biomarker of oxidative stress in human plasma: The role of lysine analogs'. Together they form a unique fingerprint.

  • Cite this