Assay for simultaneous determination of β-cyclodextrin glycosyltransferase (β-CGTase) and amylase

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A new procedure was developed for the simultaneous determination of β-cyclodextrin glycosyltransferase (β-CGTase) and amylase activities in Bacillus macerons cultures. The new amylase assay branched off from the β-CGTase activity measurement according to Kaneko et al. (J. Jpn. Soc. Starch Sci. 34 [1987], 45-48), included water-soluble starch as substrate and based on the well-characterized K3[Fe(CN)6] reducing sugar analysis procedure. The assay had excellent within-run and between-run CVs, and also had an excellent correlation with the widely used 3,5-dinitrosalicylic acid method of Bernfeld (Meth. Enzymol. 1 [1955], 149-158) using Bacillus licheniformis a-amylase as a model enzyme at 50°C. Neither high cyclodextrin glycosyltransferase activities nor the self-degradation of starch interfered with the new amylase assay.

Original languageEnglish
Pages (from-to)36-38
Number of pages3
JournalStarch/Staerke
Volume50
Issue number1
Publication statusPublished - Jan 1 1998

    Fingerprint

ASJC Scopus subject areas

  • Food Science
  • Organic Chemistry

Cite this