A new procedure was developed for the simultaneous determination of β-cyclodextrin glycosyltransferase (β-CGTase) and amylase activities in Bacillus macerons cultures. The new amylase assay branched off from the β-CGTase activity measurement according to Kaneko et al. (J. Jpn. Soc. Starch Sci. 34 , 45-48), included water-soluble starch as substrate and based on the well-characterized K3[Fe(CN)6] reducing sugar analysis procedure. The assay had excellent within-run and between-run CVs, and also had an excellent correlation with the widely used 3,5-dinitrosalicylic acid method of Bernfeld (Meth. Enzymol. 1 , 149-158) using Bacillus licheniformis a-amylase as a model enzyme at 50°C. Neither high cyclodextrin glycosyltransferase activities nor the self-degradation of starch interfered with the new amylase assay.
|Number of pages||3|
|Publication status||Published - Jan 1 1998|
ASJC Scopus subject areas
- Food Science
- Organic Chemistry