Asexual sporulation signalling regulates autolysis of Aspergillus nidulans via modulating the chitinase ChiB production

I. Pócsi, É Leiter, N. J. Kwon, K. S. Shin, G. S. Kwon, T. Pusztahelyi, T. Emri, R. A. Abuknesha, R. G. Price, J. H. Yu

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Aims: Elucidation of the regulation of ChiB production in Aspergillus nidulans. Methods and Results: Mutational inactivation of the A. nidulans chiB gene resulted in a nonautolytic phenotype. To better understand the mechanisms controlling both developmental progression and fungal autolysis, we examined a range of autolysis-associated parameters in A. nidulans developmental and / or autolytic mutants. Investigation of disorganization of mycelial pellets, loss of biomass, extra- / intracellular chitinase activities, ChiB production and chiB mRNA levels in various cultures revealed that, in submerged cultures, initialization of autolysis and stationary phase-induced ChiB production are intimately coupled, and that both processes are controlled by the FluG-BrlA asexual sporulation regulatory pathway. ChiB production does not affect the progression of apoptotic cell death in the aging A. nidulans cultures. Conclusions: The endochitinase ChiB plays an important role in autolysis of A. nidulans, and its production is initiated by FluG-BrlA signalling. Despite the fact that apoptosis is an inseparable part of fungal autolysis, its regulation is independent to FluG-initiated sporulation signalling. Significance and Impact of the Study: Deletion of chiB and fluG homologues in industrial filamentous fungal strains may stabilize the hyphal structures in the autolytic phase of growth and limit the release of autolytic hydrolases into the culture medium.

Original languageEnglish
Pages (from-to)514-523
Number of pages10
JournalJournal of applied microbiology
Volume107
Issue number2
DOIs
Publication statusPublished - Aug 1 2009

Keywords

  • Apoptosis
  • Aspergillus nidulans
  • Autolysis
  • Chitinase
  • Penicillium chrysogenum
  • Sporulation.

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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