Ascorbate oxidase-based amperometric biosensor for L-ascorbic acid determination in beverages

Gábor Csiffáry, Péter Futo, N. Adányi, Attila Kiss

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A novel biosensor for l-ascorbic acid determination in different beverages was elaborated. The ascorbate oxidase enzyme (AAO) from Cucurbita sp., EC 1.10.3.3, was immobilized on a screen-printed carbon electrode with poly(ethylene glycol) (400) diglycidyl ether (PEGDGE) as a crosslinking agent. The standards and samples were measured first with a blank electrode. An inert protein, bovine serum albumin (BSA), was immobilized on the surface of this electrode with PEGDGE. The BSA mass was equivalent to the mass of 10 U of AAO enzyme immobilized on the electrodes (0.021 mg). The linear measuring range for l-ascorbic acid was between 5 and 150 μmol/L. As l-ascorbic acid is a vital vitamin and a common antioxidant used in food industry, fruit juices and vitamin C effervescent tablets were examined. The results were compared to HPLC measurements.

Original languageEnglish
Pages (from-to)31-35
Number of pages5
JournalFood Technology and Biotechnology
Volume54
Issue number1
DOIs
Publication statusPublished - Jan 1 2016

Fingerprint

Ascorbate Oxidase
ascorbate oxidase
Beverages
biosensors
Ascorbic acid
Biosensing Techniques
Biosensors
electrodes
beverages
Ascorbic Acid
Electrodes
ascorbic acid
Enzymes
Vitamins
bovine serum albumin
Bovine Serum Albumin
polyethylene glycol
Ether
Polyethylene glycols
ethers

Keywords

  • Ascorbate oxidase
  • Biosensor
  • L-ascorbic acid
  • Screen-printed carbon electrodes

ASJC Scopus subject areas

  • Food Science
  • Industrial and Manufacturing Engineering
  • Chemical Engineering(all)
  • Biotechnology

Cite this

Ascorbate oxidase-based amperometric biosensor for L-ascorbic acid determination in beverages. / Csiffáry, Gábor; Futo, Péter; Adányi, N.; Kiss, Attila.

In: Food Technology and Biotechnology, Vol. 54, No. 1, 01.01.2016, p. 31-35.

Research output: Contribution to journalArticle

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