Ascorbate accumulation during sulphur deprivation and its effects on photosystem II activity and H2 production of the green alga Chlamydomonas reinhardtii

Valéria Nagy, André Vidal-Meireles, Roland Tengölics, G. Rákhely, G. Garab, László Kovács, Szilvia Z. Tóth

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In nature, H2 production in Chlamydomonas reinhardtii serves as a safety valve during the induction of photosynthesis in anoxia, and it prevents the over-reduction of the photosynthetic electron transport chain. Sulphur deprivation of C.reinhardtii also triggers a complex metabolic response resulting in the induction of various stress-related genes, down-regulation of photosynthesis, the establishment of anaerobiosis and expression of active hydrogenase. Photosystem II (PSII) plays dual role in H2 production because it supplies electrons but the evolved O2 inhibits the hydrogenase. Here, we show that upon sulphur deprivation, the ascorbate content in C.reinhardtii increases about 50-fold, reaching the mM range; at this concentration, ascorbate inactivates the Mn-cluster of PSII, and afterwards, it can donate electrons to tyrozin Z+ at a slow rate. This stage is followed by donor-side-induced photoinhibition, leading to the loss of charge separation activity in PSII and reaction centre degradation. The time point at which maximum ascorbate concentration is reached in the cell is critical for the establishment of anaerobiosis and initiation of H2 production. We also show that ascorbate influenced H2 evolution via altering the photosynthetic electron transport rather than hydrogenase activity and starch degradation.

Original languageEnglish
JournalPlant, Cell and Environment
DOIs
Publication statusAccepted/In press - 2016

Fingerprint

Hydrogenase
Chlamydomonas reinhardtii
hydrogen production
Chlorophyta
Photosystem II Protein Complex
Sulfur
photosystem II
Anaerobiosis
sulfur
anaerobiosis
Photosynthesis
Electron Transport
electrons
photosynthesis
Electrons
degradation
electron transport chain
photoinhibition
Starch
electron transfer

Keywords

  • Chlorophyll a fluorescence
  • Hydrogenase
  • Oxygen evolution
  • Thermoluminescence

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

Ascorbate accumulation during sulphur deprivation and its effects on photosystem II activity and H2 production of the green alga Chlamydomonas reinhardtii. / Nagy, Valéria; Vidal-Meireles, André; Tengölics, Roland; Rákhely, G.; Garab, G.; Kovács, László; Tóth, Szilvia Z.

In: Plant, Cell and Environment, 2016.

Research output: Contribution to journalArticle

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