Arsenic speciation in xylem sap of cucumber (Cucumis sativus L.)

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Abstract

Flow injection analysis (FIA) and high-performance liquid chromatography double-focusing sector field inductively coupled plasma mass spectrometry (HPLC-DF-ICP-MS) were used for total arsenic determination and arsenic speciation of xylem sap of cucumber plants (Cucumis sativus L.) grown in hydroponics containing 2 μmol dm-3 arsenate or arsenite, respectively. Arsenite [As(III)], arsenate [As(V)] and dimethylarsinic acid (DMA) were identified in the sap of the plants. Arsenite was the predominant arsenic species in the xylem saps regardless of the type of arsenic treatment, and the following concentration order was determined: As(III) > As(V) > DMA. The amount of total As, calculated taking into consideration the mass of xylem sap collected, was almost equal for both treatments. Arsenite was taken up more easily by cucumber than arsenate. Partial oxidation of arsenite to arsenate (<10% in 48 h) was observed in the case of arsenite-containing nutrient solutions, which may explain the detection of arsenate in the saps of plants treated with arsenite.

Original languageEnglish
Pages (from-to)461-466
Number of pages6
JournalAnalytical and Bioanalytical Chemistry
Volume383
Issue number3
DOIs
Publication statusPublished - Oct 1 2005

Keywords

  • Arsenic
  • HPLC
  • ICP-MS
  • Plants
  • Speciation
  • Xylem

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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