Aromatic stacking between nucleobase and enzyme promotes phosphate ester hydrolysis in dUTPase

Ildiko Pecsi, Ibolya Leveles, Veronika Harmat, Beata G. Vertessy, Judit Toth

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Aromatic interactions are well-known players in molecular recognition but their catalytic role in biological systems is less documented. Here, we report that a conserved aromatic stacking interaction between dUTPase and its nucleotide substrate largely contributes to the stabilization of the associative type transition state of the nucleotide hydrolysis reaction. The effect of the aromatic stacking on catalysis is peculiar in that uracil, the aromatic moiety influenced by the aromatic interaction is relatively distant from the site of hydrolysis at the alpha-phosphate group. Using crystallographic, kinetics, optical spectroscopy and thermodynamics calculation approaches we delineate a possible mechanism by which rate acceleration is achieved through the remote n-n interaction. The abundance of similarly positioned aromatic interactions in various nucleotide hydrolyzing enzymes (e.g. most families of ATPases) raises the possibility of the reported phenomenon being a general component of the enzymatic catalysis of phosphate ester hydrolysis.

Original languageEnglish
Pages (from-to)7179-7186
Number of pages8
JournalNucleic acids research
Volume38
Issue number20
DOIs
Publication statusPublished - Nov 1 2010

    Fingerprint

ASJC Scopus subject areas

  • Genetics

Cite this