Arginine methylation provides epigenetic transcription memory for retinoid-induced differentiation in myeloid cells

Balint L. Balint, Attila Szanto, A. Mádi, Uta Maria Bauer, Petra Gabor, Sz. Benkő, L. Puskás, Peter J A Davies, L. Nagy

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Cellular differentiation is governed by changes in gene expression, but at the same time, a cell's identity needs to be maintained through multiple cell divisions during maturation. In myeloid cell lines, retinoids induce gene expression and a well-characterized two-step lineage-specific differentiation. To identify mechanisms that contribute to cellular transcriptional memory, we analyzed the epigenetic changes taking place on regulatory regions of tissue transglutaminase, a gene whose expression is tightly linked to retinoid-induced differentiation. Here we report that the induction of an intermediary or "primed" state of myeloid differentiation is associated with increased H4 arginine 3 and decreased H3 lysine 4 methylation. These modifications occur before transcription and appear to prime the chromatin for subsequent hormone-regulated transcription. Moreover, inhibition of methyltransferase activity, preacetylation, or activation of the enzyme PAD4 attenuated retinoid-regulated gene expression, while overexpression of PRMT1, a methyltransferase, enhanced retinoid responsiveness. Taken together, our results suggest that H4 arginine 3 methylation is a bona fide positive epigenetic marker and regulator of transcriptional responsiveness as well as a signal integration mechanism during cell differentiation and, as such, may provide epigenetic memory.

Original languageEnglish
Pages (from-to)5648-5663
Number of pages16
JournalMolecular and Cellular Biology
Volume25
Issue number13
DOIs
Publication statusPublished - Jul 2005

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Retinoids
Myeloid Cells
Epigenomics
Methylation
Arginine
Gene Expression
Methyltransferases
Enzyme Activation
Nucleic Acid Regulatory Sequences
Cell Division
Lysine
Chromatin
Cell Differentiation
Hormones
Cell Line

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Arginine methylation provides epigenetic transcription memory for retinoid-induced differentiation in myeloid cells. / Balint, Balint L.; Szanto, Attila; Mádi, A.; Bauer, Uta Maria; Gabor, Petra; Benkő, Sz.; Puskás, L.; Davies, Peter J A; Nagy, L.

In: Molecular and Cellular Biology, Vol. 25, No. 13, 07.2005, p. 5648-5663.

Research output: Contribution to journalArticle

Balint, Balint L. ; Szanto, Attila ; Mádi, A. ; Bauer, Uta Maria ; Gabor, Petra ; Benkő, Sz. ; Puskás, L. ; Davies, Peter J A ; Nagy, L. / Arginine methylation provides epigenetic transcription memory for retinoid-induced differentiation in myeloid cells. In: Molecular and Cellular Biology. 2005 ; Vol. 25, No. 13. pp. 5648-5663.
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