Areal and subcellular localization of the ubiquitous alkaline phosphatase in the primate cerebral cortex

Evidence for a role in neurotransmission

Caroline Fonta, L. Négyessy, Luc Renaud, Pascal Barone

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

The ubiquitous enzyme TNAP (tissue non-specific alkaline phosphatase) is found in numerous tissues such as liver, kidney and bone, but little attention has been paid to its expression and role in the brain. Observations in TNAP-KO mice, which analyzed the role of this enzyme in osteogenesis, had suggested that TNAP might be involved in GABA neurotransmission. Apart from its presence in endothelial cells, here we show a specific and strong alkaline phosphatase (AP) activity in the neuropile, matching the pattern of thalamo-cortical innervation in layer 4 of the primate sensory cortices (visual, auditory and somatosensory). Such a pattern is also evident in rodents and carnivores, making AP a powerful marker of primary sensory areas. Remarkably, AP activity is regulated by sensory experience as demonstrated by monocular deprivation paradigms in monkeys. The areal and laminar distribution of AP activity matches that of the GAD65, the GABA synthesizing enzyme found in presynatic terminals. As our electron microscopic investigations indicate that AP is found at the neuronal membranes and in synaptic contacts, it is proposed that the neuronal AP isoform (NAP), may be a key enzyme in regulating neurotransmission and could therefore play an important role in developmental plasticity and activity-dependent cortical functions.

Original languageEnglish
Pages (from-to)595-609
Number of pages15
JournalCerebral Cortex
Volume14
Issue number6
DOIs
Publication statusPublished - Jun 2004

Fingerprint

Synaptic Transmission
Cerebral Cortex
Primates
Alkaline Phosphatase
Enzymes
gamma-Aminobutyric Acid
Synaptic Membranes
Auditory Cortex
Somatosensory Cortex
Neuropil
Visual Cortex
Osteogenesis
Haplorhini
Rodentia
Protein Isoforms
Endothelial Cells
Electrons
Kidney
Bone and Bones
Liver

Keywords

  • GABA
  • Monkey
  • Pyridoxal 5′phosphate
  • Sensory cortex
  • TNAP

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Areal and subcellular localization of the ubiquitous alkaline phosphatase in the primate cerebral cortex : Evidence for a role in neurotransmission. / Fonta, Caroline; Négyessy, L.; Renaud, Luc; Barone, Pascal.

In: Cerebral Cortex, Vol. 14, No. 6, 06.2004, p. 595-609.

Research output: Contribution to journalArticle

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