Arachidonic acid activatable electrogenic H+ transport in the absence of cytochrome b558 in human T lymphocytes

Krisztina Káldi, Katalin Szászi, Gábor Koncz, Katalin Suszták, Erzsébet Ligeti

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12 Citations (Scopus)

Abstract

To test the suggested structural relationship between the electrogenic H+ transporting system and the NADPH oxidase of phagocytes, the existence of the enzyme and the transport process was investigated in human tonsillar T lymphocytes. It is shown that tonsillar T cells possess an arachidonic acid activatable, Cd2+-and Zn2+-sensitive electrogenic H- efflux pathway with similar properties as reported earlier in various phagocytic cells. The presence of cytochrome b558, the membrane component of the oxidase, could not be detected in tonsillar T lymphocytes either by immunoblot or by flow cytometric analysis. It is suggested that the electrogenic H+ transporting pathway is structurally independent of the NADPH oxidase complex.

Original languageEnglish
Pages (from-to)156-160
Number of pages5
JournalFEBS letters
Volume381
Issue number1-2
DOIs
Publication statusPublished - Feb 26 1996

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Keywords

  • Arachidonic acid
  • Electrogenic H-transporter
  • NADPH oxidase
  • Neutrophil granulocyte
  • Phospholipase A
  • T lymphocyte

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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