Arabidopsis PPR40 connects abiotic stress responses to mitochondrial electron transport

Laura Zsigmond, Gábor Rigó, A. Szarka, Gyöngyi Székely, Krisztina Ötvös, Z. Darula, Katalin F. Medzihradszky, Csaba Koncz, Zsuzsa Koncz, L. Szabados

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Oxidative respiration produces adenosine triphosphate through the mitochondrial electron transport system controlling the energy supply of plant cells. Here we describe a mitochondrial pentatricopeptide repeat (PPR) domain protein, PPR40, which provides a signaling link between mitochondrial electron transport and regulation of stress and hormonal responses in Arabidopsis (Arabidopsis thaliana). Insertion mutations inactivating PPR40 result in semidwarf growth habit and enhanced sensitivity to salt, abscisic acid, and oxidative stress. Genetic complementation by overexpression of PPR40 complementary DNA restores the ppr40 mutant phenotype to wild type. The PPR40 protein is localized in the mitochondria and found in association with Complex III of the electron transport system. In the ppr40-1 mutant the electron transport through Complex III is strongly reduced, whereas Complex IV is functional, indicating that PPR40 is important for the ubiqinol-cytochrome c oxidoreductase activity of Complex III. Enhanced stress sensitivity of the ppr40-1 mutant is accompanied by accumulation of reactive oxygen species, enhanced lipid peroxidation, higher superoxide dismutase activity, and altered activation of several stress-responsive genes including the alternative oxidase AOX1d. These results suggest a close link between regulation of oxidative respiration and environmental adaptation in Arabidopsis.

Original languageEnglish
Pages (from-to)1721-1737
Number of pages17
JournalPlant Physiology
Volume146
Issue number4
DOIs
Publication statusPublished - Apr 2008

Fingerprint

Electron Transport Complex III
ubiquinol-cytochrome-c reductase
Electron Transport
Arabidopsis
abiotic stress
electron transfer
stress response
cell respiration
mutants
Respiration
genetic complementation
Abscisic Acid
complementary DNA
adenosine triphosphate
Insertional Mutagenesis
cytochrome c
oxidoreductases
Plant Cells
growth habit
Cytochromes c

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Arabidopsis PPR40 connects abiotic stress responses to mitochondrial electron transport. / Zsigmond, Laura; Rigó, Gábor; Szarka, A.; Székely, Gyöngyi; Ötvös, Krisztina; Darula, Z.; Medzihradszky, Katalin F.; Koncz, Csaba; Koncz, Zsuzsa; Szabados, L.

In: Plant Physiology, Vol. 146, No. 4, 04.2008, p. 1721-1737.

Research output: Contribution to journalArticle

Zsigmond, L, Rigó, G, Szarka, A, Székely, G, Ötvös, K, Darula, Z, Medzihradszky, KF, Koncz, C, Koncz, Z & Szabados, L 2008, 'Arabidopsis PPR40 connects abiotic stress responses to mitochondrial electron transport', Plant Physiology, vol. 146, no. 4, pp. 1721-1737. https://doi.org/10.1104/pp.107.111260
Zsigmond, Laura ; Rigó, Gábor ; Szarka, A. ; Székely, Gyöngyi ; Ötvös, Krisztina ; Darula, Z. ; Medzihradszky, Katalin F. ; Koncz, Csaba ; Koncz, Zsuzsa ; Szabados, L. / Arabidopsis PPR40 connects abiotic stress responses to mitochondrial electron transport. In: Plant Physiology. 2008 ; Vol. 146, No. 4. pp. 1721-1737.
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