Approach for ochratoxin A fast screening in spices using clean-up tandem immunoassay columns with confirmation by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)

I. Yu Goryacheva, S. De Saeger, M. Lobeau, S. A. Eremin, I. Barna-Vetró, C. Van Peteghem

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

An approach for ochratoxin A (OTA) fast cost-effective screening based on clean-up tandem immunoassay columns was developed and optimized for OTA detection with a cut-off level of 10 μg kg-1 in spices. Two procedures were tested and applied for OTA detection. Column with bottom detection immunolayer was optimized for OTA determination in Capsicum ssp. spices. A modified clean-up tandem immunoassay procedure with top detection immunolayer was successfully applied for all tested spices. Its main advantages were decreasing of the number of analysis steps and quantity of antibody and also minimizing of matrix effects. The total duration of the extraction and analysis was about 40 min for six samples. Chilli, red pepper, pili-pili, cayenne, paprika, nutmeg, ginger, white pepper and black pepper samples were analyzed for OTA contamination by the proposed clean-up tandem immunoassay procedures. Clean-up tandem immunoassay results were confirmed by HPLC-MS/MS with immunoaffinity column clean-up. Among 17 tested Capsicum ssp. spices, 6 samples (35%) contained OTA in a concentration exceeding the 10 μg kg-1 limit discussed by the European Commission. All tested nutmeg (n = 8), ginger (n = 5), white pepper (n = 7) and black pepper (n = 6) samples did not contain OTA above this action level.

Original languageEnglish
Pages (from-to)38-45
Number of pages8
JournalAnalytica Chimica Acta
Volume577
Issue number1
DOIs
Publication statusPublished - Sep 1 2006

Fingerprint

spice
Spices
immunoassay
High performance liquid chromatography
Capsicum
Tandem Mass Spectrometry
Immunoassay
Piper nigrum
Mass spectrometry
liquid chromatography
Screening
mass spectrometry
High Pressure Liquid Chromatography
Myristica fragrans
Ginger
European Commission
antibody
matrix
ochratoxin A
screening

Keywords

  • High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS)
  • Immunoassay
  • Ochratoxin A
  • Screening
  • Spices

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

Approach for ochratoxin A fast screening in spices using clean-up tandem immunoassay columns with confirmation by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). / Goryacheva, I. Yu; De Saeger, S.; Lobeau, M.; Eremin, S. A.; Barna-Vetró, I.; Van Peteghem, C.

In: Analytica Chimica Acta, Vol. 577, No. 1, 01.09.2006, p. 38-45.

Research output: Contribution to journalArticle

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