Appraisal of the potassium ferricyanide method for the demonstration of glycogen granules by electron microscopy

M. Boldoczki, A. Párducz, F. Joo

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Work has been carried out to determine whether the presence of iron following the use of De Bruijn's potassium ferricyanide method is specific for glycogen granules. Samples of rat liver were processed according to De Bruijn's method and also without the addition of the ferricyanide, dehydrated, embedded in Durcupan and thick (150-200 nm) sections analyzed using an EDAX X-ray spectrometer attached to a JEOL 100 B transmission electron microscope. Iron was not detected in the tissue not treated with the ferricyanide. However, in tissue processed according to De Bruijn's method it was not only detected in regions rich in glycogen granules but also in approximately similar amounts (as judged by peak height) in regions containing granular endoplasmic reticulum and in mitochondria. Iron was also detected in mitochondria of tissue subjected to diastase extraction. It is apparent that the presence of iron is not specific for glycogen granules as a morphological entity although their electron-optical density is increased. The possible reasons for the presence of iron in other cytoplasmic components is discussed; while it may reflect a non-specific result histochemically it is possible that it may be due to the presence of glycogen residues or complexes, some of which may be relatively resistant to diastase extraction.

Original languageEnglish
Pages (from-to)201-206
Number of pages6
JournalMicron (1969)
Volume8
Issue number4
DOIs
Publication statusPublished - 1978

Fingerprint

glycogens
Glycogen
Electron microscopy
Potassium
potassium
electron microscopy
Electron Microscopy
Demonstrations
Iron
iron
Mitochondria
mitochondria
Amylases
Tissue
Electrons
X ray spectrometers
endoplasmic reticulum
Density (optical)
Rough Endoplasmic Reticulum
optical density

ASJC Scopus subject areas

  • Cell Biology
  • Materials Science(all)
  • Instrumentation

Cite this

Appraisal of the potassium ferricyanide method for the demonstration of glycogen granules by electron microscopy. / Boldoczki, M.; Párducz, A.; Joo, F.

In: Micron (1969), Vol. 8, No. 4, 1978, p. 201-206.

Research output: Contribution to journalArticle

@article{d35e8943badf4242ac58327388ac7733,
title = "Appraisal of the potassium ferricyanide method for the demonstration of glycogen granules by electron microscopy",
abstract = "Work has been carried out to determine whether the presence of iron following the use of De Bruijn's potassium ferricyanide method is specific for glycogen granules. Samples of rat liver were processed according to De Bruijn's method and also without the addition of the ferricyanide, dehydrated, embedded in Durcupan and thick (150-200 nm) sections analyzed using an EDAX X-ray spectrometer attached to a JEOL 100 B transmission electron microscope. Iron was not detected in the tissue not treated with the ferricyanide. However, in tissue processed according to De Bruijn's method it was not only detected in regions rich in glycogen granules but also in approximately similar amounts (as judged by peak height) in regions containing granular endoplasmic reticulum and in mitochondria. Iron was also detected in mitochondria of tissue subjected to diastase extraction. It is apparent that the presence of iron is not specific for glycogen granules as a morphological entity although their electron-optical density is increased. The possible reasons for the presence of iron in other cytoplasmic components is discussed; while it may reflect a non-specific result histochemically it is possible that it may be due to the presence of glycogen residues or complexes, some of which may be relatively resistant to diastase extraction.",
author = "M. Boldoczki and A. P{\'a}rducz and F. Joo",
year = "1978",
doi = "10.1016/0047-7206(78)90058-4",
language = "English",
volume = "8",
pages = "201--206",
journal = "Micron (1969)",
issn = "0047-7206",
publisher = "Pergamon Press Ltd.",
number = "4",

}

TY - JOUR

T1 - Appraisal of the potassium ferricyanide method for the demonstration of glycogen granules by electron microscopy

AU - Boldoczki, M.

AU - Párducz, A.

AU - Joo, F.

PY - 1978

Y1 - 1978

N2 - Work has been carried out to determine whether the presence of iron following the use of De Bruijn's potassium ferricyanide method is specific for glycogen granules. Samples of rat liver were processed according to De Bruijn's method and also without the addition of the ferricyanide, dehydrated, embedded in Durcupan and thick (150-200 nm) sections analyzed using an EDAX X-ray spectrometer attached to a JEOL 100 B transmission electron microscope. Iron was not detected in the tissue not treated with the ferricyanide. However, in tissue processed according to De Bruijn's method it was not only detected in regions rich in glycogen granules but also in approximately similar amounts (as judged by peak height) in regions containing granular endoplasmic reticulum and in mitochondria. Iron was also detected in mitochondria of tissue subjected to diastase extraction. It is apparent that the presence of iron is not specific for glycogen granules as a morphological entity although their electron-optical density is increased. The possible reasons for the presence of iron in other cytoplasmic components is discussed; while it may reflect a non-specific result histochemically it is possible that it may be due to the presence of glycogen residues or complexes, some of which may be relatively resistant to diastase extraction.

AB - Work has been carried out to determine whether the presence of iron following the use of De Bruijn's potassium ferricyanide method is specific for glycogen granules. Samples of rat liver were processed according to De Bruijn's method and also without the addition of the ferricyanide, dehydrated, embedded in Durcupan and thick (150-200 nm) sections analyzed using an EDAX X-ray spectrometer attached to a JEOL 100 B transmission electron microscope. Iron was not detected in the tissue not treated with the ferricyanide. However, in tissue processed according to De Bruijn's method it was not only detected in regions rich in glycogen granules but also in approximately similar amounts (as judged by peak height) in regions containing granular endoplasmic reticulum and in mitochondria. Iron was also detected in mitochondria of tissue subjected to diastase extraction. It is apparent that the presence of iron is not specific for glycogen granules as a morphological entity although their electron-optical density is increased. The possible reasons for the presence of iron in other cytoplasmic components is discussed; while it may reflect a non-specific result histochemically it is possible that it may be due to the presence of glycogen residues or complexes, some of which may be relatively resistant to diastase extraction.

UR - http://www.scopus.com/inward/record.url?scp=0017591411&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0017591411&partnerID=8YFLogxK

U2 - 10.1016/0047-7206(78)90058-4

DO - 10.1016/0047-7206(78)90058-4

M3 - Article

VL - 8

SP - 201

EP - 206

JO - Micron (1969)

JF - Micron (1969)

SN - 0047-7206

IS - 4

ER -