Application of two-photon microscopy to the study of cellular pharmacology of central neurons

B. Lendvai, S. I. Szabo, A. I. Barth, T. Zelles, E. Vízi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Two-photon microscopy is an especially powerful tool for combining anatomical and physiological experiments in the central nervous system: the possibility of simultaneously studying physiological phenomena in well-defined anatomical compartments allows fluorescence imaging of neurons in deeper layers of the brain. In this review we summarize the most commonly used brain preparation techniques together with the methods of loading neurons with fluorescent indicators. We will focus primarily on issues of drug delivery specifically related to two-photon experiments highlighting the different ways of drug administration. Methods of chemical stimulation via caged neurotransmitters are also discussed. Finally a few specific areas of two-photon applications in drug research on neuronal tissue are highlighted.

Original languageEnglish
Pages (from-to)841-849
Number of pages9
JournalAdvanced Drug Delivery Reviews
Volume58
Issue number7
DOIs
Publication statusPublished - Oct 31 2006

Fingerprint

Photons
Microscopy
Pharmacology
Neurons
Pharmaceutical Preparations
Chemical Stimulation
Physiological Phenomena
Optical Imaging
Brain
Neurotransmitter Agents
Central Nervous System
Research

Keywords

  • Bath perfusion
  • Brain preparation
  • Brain slice
  • Cellular pharmacology
  • Organotypic slice culture
  • Pressure ejection
  • Two-photon microscopy
  • Uncaging

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Application of two-photon microscopy to the study of cellular pharmacology of central neurons. / Lendvai, B.; Szabo, S. I.; Barth, A. I.; Zelles, T.; Vízi, E.

In: Advanced Drug Delivery Reviews, Vol. 58, No. 7, 31.10.2006, p. 841-849.

Research output: Contribution to journalArticle

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